Effects of low omega-6/omega-3 ratio and high omega-9/omega-6 ratio oil blend on inflammation and oxidative stress after tooth extraction in rats.

IF 5.3 2区医学 Q2 IMMUNOLOGY

Inflammopharmacology Pub Date : 2025-09-09 DOI:10.1007/s10787-025-01892-x

Radamés Bezerra Melo, Gabriella Alves Julião Costa, Paulo Goberlânio de Barros Silva, Reinaldo Barreto Oriá, Cristhyane Costa de Aquino, Paulo Roberto Leitão Vasconcelos

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引用次数: 0

Abstract

Objective: This study evaluated the effects and mechanisms of antioxidant and anti-inflammatory oils with a high omega-9:omega-6 ratio and a low omega-6:omega-3 ratio on post-extraction healing in rats.

Materials and methods: A total of 128 Wistar rats were divided into four groups: Sham, Saline, Isolipidic, and Anti-inflammatory/Antioxidant. The animals received one of the following treatments: (1) 0.9% NaCl solution (Sham Group or Saline Group); (2) an isolipidic mixture containing alpha-linolenic acid (ALA) and a combination of ω-9, ω-6, and ω-3 fatty acids derived from corn oil and soybean oil-rich in omega-6:omega-3 and omega-9:omega-6 ratios (Isolipidic Group); or (3) an anti-inflammatory and antioxidant mixture containing ALA, docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA), composed of olive oil (61%), canola oil (24%), and fish oil (15%) (Test Group). Treatments were administered by gavage for four days pre-extraction and three days post-surgery. Animals were euthanized at 3, 7, 14, and 21 days post-extraction. Blood and tissue samples were analyzed for myeloperoxidase, osteoclast count, IL-1β, NFκB, iNOS, BMP-2 expression, glutathione (GSH), and thiobarbituric acid reactive substances (TBARS).

Results: The Anti-inflammatory/Antioxidant mixture significantly reduced myeloperoxidase expression, osteoclast count, GSH, TBARS, and IL-1β levels on day 7. It also decreased NFκB, iNOS, and BMP-2 expression on days 3 and 7 compared to the Saline and Isolipidic groups.

Conclusion: The Anti-inflammatory and Antioxidant mixture effectively reduced inflammation and oxidative stress during the inflammatory and proliferative phases of post-extraction healing, suggesting potential benefits in clinical applications for oral surgery recovery.

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低omega-6/omega-3比例和高omega-9/omega-6比例混合油对大鼠拔牙后炎症和氧化应激的影响。

目的：研究高-9:-6比例和低-6:-3比例的抗氧化和抗炎油对大鼠提取后愈合的影响及其机制。材料与方法：将128只Wistar大鼠分为4组：假手术组、生理盐水组、等脂组、抗炎/抗氧化组。各组分别给予0.9% NaCl溶液（Sham组或生理盐水组）；(2)含有α -亚麻酸（ALA）和ω-9、ω-6和ω-3脂肪酸的组合的等脂类混合物，这些脂肪酸来源于富含ω-6: ω-3和ω-9: ω-6比率的玉米油和大豆油（等脂类）；或(3)含有ALA、二十二碳六烯酸（DHA）和二十碳五烯酸（EPA）的抗炎和抗氧化混合物，由橄榄油（61%）、菜籽油（24%）和鱼油（15%）组成（试验组）。拔牙前4天、术后3天灌胃治疗。分别于拔牙后3、7、14和21天对动物实施安乐死。分析血液和组织样本的髓过氧化物酶、破骨细胞计数、IL-1β、NFκB、iNOS、BMP-2表达、谷胱甘肽（GSH）和硫代巴比妥酸反应物质（TBARS）。结果：抗炎/抗氧化混合物在第7天显著降低髓过氧化物酶表达、破骨细胞计数、GSH、TBARS和IL-1β水平。与生理盐水组和等脂体组相比，在第3天和第7天，它还降低了NFκB、iNOS和BMP-2的表达。结论：抗炎抗氧化合剂可有效降低拔牙后炎症期和增殖期的炎症和氧化应激，具有临床应用价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Inflammopharmacology IMMUNOLOGYTOXICOLOGY-TOXICOLOGY

CiteScore

8.00

自引率

3.40%

发文量

200

期刊介绍： Inflammopharmacology is the official publication of the Gastrointestinal Section of the International Union of Basic and Clinical Pharmacology (IUPHAR) and the Hungarian Experimental and Clinical Pharmacology Society (HECPS). Inflammopharmacology publishes papers on all aspects of inflammation and its pharmacological control emphasizing comparisons of (a) different inflammatory states, and (b) the actions, therapeutic efficacy and safety of drugs employed in the treatment of inflammatory conditions. The comparative aspects of the types of inflammatory conditions include gastrointestinal disease (e.g. ulcerative colitis, Crohn''s disease), parasitic diseases, toxicological manifestations of the effects of drugs and environmental agents, arthritic conditions, and inflammatory effects of injury or aging on skeletal muscle. The journal has seven main interest areas: -Drug-Disease Interactions - Conditional Pharmacology - i.e. where the condition (disease or stress state) influences the therapeutic response and side (adverse) effects from anti-inflammatory drugs. Mechanisms of drug-disease and drug disease interactions and the role of different stress states -Rheumatology - particular emphasis on methods of measurement of clinical response effects of new agents, adverse effects from anti-rheumatic drugs -Gastroenterology - with particular emphasis on animal and human models, mechanisms of mucosal inflammation and ulceration and effects of novel and established anti-ulcer, anti-inflammatory agents, or antiparasitic agents -Neuro-Inflammation and Pain - model systems, pharmacology of new analgesic agents and mechanisms of neuro-inflammation and pain -Novel drugs, natural products and nutraceuticals - and their effects on inflammatory processes, especially where there are indications of novel modes action compared with conventional drugs e.g. NSAIDs -Muscle-immune interactions during inflammation [...]