High-Yield Isolation of Stem Cell-Derived Extracellular Vesicles Using a Gold Nanoparticle-Enhanced SiO₂ Immunoaffinity Platform.

IF 4.4 4区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

IEEE Transactions on NanoBioscience Pub Date : 2025-09-08 DOI:10.1109/TNB.2025.3606977

Krishna Thej Pammi Guru, Nusrat Praween, Palash Kumar Basu

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引用次数: 0

Abstract

Extracellular vesicles (EVs) produced by stem cells are nanoscale carriers of bioactive compounds with regenerative and immunomodulatory capabilities similar to those of their parent cells. Their therapeutic potential outperforms traditional stem cell therapies by lowering hazards such tumorigenicity and allowing for precise delivery. To provide a high-efficiency platform for selectively isolating stem cell EVs from minimal serum quantities while overcoming the constraints of traditional approaches such as ultracentrifugation, we developed an immunoaffinity-based capture system utilizing SiO₂ wafers functionalized with gold nanoparticles (GNPs), polyethylene glycol (HS-PEG-COOH), and stem cell-specific antibodies. The platform was evaluated to isolate EVs from 20 μL serum samples. The technique efficiently and selectively isolates EVs, including stem cell-derived subtypes, with yields of up to 10⁸ particles. Western blot testing demonstrated high purity and low protein contamination, demonstrating the capture mechanism's selectivity. This nanoparticle-enhanced platform allows for scalable, high-purity EV extraction from small sample volumes, which aids in downstream molecular analysis and therapeutic development. Its capacity to distinguish across EV subtypes has potential in personalized medicine, regenerative therapies, and non-invasive diagnostics.

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利用金纳米颗粒增强sio2免疫亲和平台高效分离干细胞来源的细胞外囊泡。

干细胞产生的细胞外囊泡（EVs）是生物活性化合物的纳米级载体，具有与其亲本细胞相似的再生和免疫调节能力。它们的治疗潜力优于传统的干细胞疗法，因为它们降低了致瘤性等危险，并允许精确递送。为了提供一个从少量血清中选择性分离干细胞ev的高效平台，同时克服传统方法（如超离心）的限制，我们开发了一种基于免疫亲和的捕获系统，该系统利用金纳米颗粒（GNPs）、聚乙二醇（HS-PEG-COOH）和干细胞特异性抗体功能化的SiO₂晶片。对该平台在20 μL血清样品中分离ev的能力进行了评价。该技术有效且有选择性地分离出ev，包括干细胞衍生亚型，产量高达10⁸颗粒。Western blot检测结果表明，该蛋白纯度高，蛋白污染低，具有一定的选择性。这种纳米粒子增强平台允许从小样本量中提取可扩展的高纯度EV，这有助于下游分子分析和治疗开发。其区分EV亚型的能力在个性化医疗、再生治疗和非侵入性诊断方面具有潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

IEEE Transactions on NanoBioscience 工程技术-纳米科技

CiteScore

7.00

自引率

5.10%

发文量

197

审稿时长

>12 weeks

期刊介绍： The IEEE Transactions on NanoBioscience reports on original, innovative and interdisciplinary work on all aspects of molecular systems, cellular systems, and tissues (including molecular electronics). Topics covered in the journal focus on a broad spectrum of aspects, both on foundations and on applications. Specifically, methods and techniques, experimental aspects, design and implementation, instrumentation and laboratory equipment, clinical aspects, hardware and software data acquisition and analysis and computer based modelling are covered (based on traditional or high performance computing - parallel computers or computer networks).