Zoe Z Chen, Sujanya N Narayanan, Lena M Stagliano, Peter Q Huynh, Shivani Sundaram, Emma J Mackey, Craig T Miller, Tyler A Square
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引用次数: 0
Abstract
Organ initiation is often driven by extracellular signaling molecules that activate precursor cells competent to receive and respond to a given signal, yet little is known about the dynamics of competency in space and time during development. Teeth are excellent model organs for studying cellular competency because they can be activated with the addition of a single signaling ligand, Ectodysplasin (Eda). To investigate the role of Eda in tooth specification, we generated transgenic sticklebacks and zebrafish with heat shock-inducible Eda overexpression. We found that stickleback Eda can drive ectopic tooth formation in at least eight distinct morphological domains. Both zebrafish and stickleback exhibit maximal responsiveness to Eda overexpression during pioneer tooth initiation. Analysis of candidate receptor expression in sticklebacks reveals that ectopic tooth formation in the pharynx correlates with Edar and Troy expression, while only Troy expression was detected in regions of the face where teeth can form, suggesting that competency may involve spatially restricted receptor expression. These findings underscore the latent developmental potential, i.e. competency, of the vertebrate dentition and provide insights into organ competency during embryonic and post-embryonic development.
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Development’s scope covers all aspects of plant and animal development, including stem cell biology and regeneration. The single most important criterion for acceptance in Development is scientific excellence. Research papers (articles and reports) should therefore pose and test a significant hypothesis or address a significant question, and should provide novel perspectives that advance our understanding of development. We also encourage submission of papers that use computational methods or mathematical models to obtain significant new insights into developmental biology topics. Manuscripts that are descriptive in nature will be considered only when they lay important groundwork for a field and/or provide novel resources for understanding developmental processes of broad interest to the community.
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