Xia Zhao, Long Li, Shan Jin, Yufei Feng, Yinfei Lu, Yankai Xu, Shiwei Xie, Lijuan Pang, Kejian Liu
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引用次数: 0
Abstract
Background: The lncRNA-miRNA-mRNA regulatory network is recognized for its significant role in cardiovascular diseases, yet its involvement in in-stent restenosis (ISR) remains unexplored. Our study aimed to investigate how this regulatory network influences ISR occurrence and development by modulating inflammation and immunity.
Methods: By utilizing data extracted from the Gene Expression Omnibus (GEO) database, we constructed the lncRNA-miRNA-mRNA regulatory network specific to ISR. We constructed a prediction model using least absolute shrinkage and selection operator (LASSO) analysis and validated it using a nomogram and receiver operating characteristic (ROC) curve analysis. For the top differentially expressed genes (DEGs), we performed functional enrichment analyses, including Gene Ontology (GO) biological processes, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, and constructed a protein-protein interaction (PPI) network. Additionally, we employed CIBERSORT to perform immunoinfiltration analysis to elucidate the relationship between DEG and immune cells.
Results: CAPN1 was identified as the optimal mRNA, corresponding to the top DEG. We developed a diagnostic prediction model with an area under the receiver operating characteristic curve (AUC) of 0.93333, indicating robust predictive performance of the model. Subsequently, the results of GO and KEGG indicated that CAPN1-related co-expression genes were predominantly enriched in the PI3K-Akt signaling pathway, with GSK3B and BRCA1 identified as key nodes within the PPI network. Additionally, we employed CIBERSORT to perform immunoinfiltration analysis to elucidate the relationship between CAPN1 and immune cells.
Conclusions: CAPN1 has the potential to function as a non-invasive biomarker for the diagnosis of ISR, which influences Tfh and naive B cells via the PI3K/AKT pathway, potentially aiding early diagnosis, treatment, and prognosis of ISR.
期刊介绍：
Clinical Laboratory is an international fully peer-reviewed journal covering all aspects of laboratory medicine and transfusion medicine. In addition to transfusion medicine topics Clinical Laboratory represents submissions concerning tissue transplantation and hematopoietic, cellular and gene therapies. The journal publishes original articles, review articles, posters, short reports, case studies and letters to the editor dealing with 1) the scientific background, implementation and diagnostic significance of laboratory methods employed in hospitals, blood banks and physicians'' offices and with 2) scientific, administrative and clinical aspects of transfusion medicine and 3) in addition to transfusion medicine topics Clinical Laboratory represents submissions concerning tissue transplantation and hematopoietic, cellular and gene therapies.
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