Prevalence of porcine circoviruses (PCV2 and PCV3) in slaughtered pigs with different pleurisy lesions score: Coinfections with Mycoplasma hyopneumoniae, Actinobacillus pleuropneumoniae, and Pasteurella multocida

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science Pub Date : 2025-09-05 DOI:10.1016/j.rvsc.2025.105892

Eduarda Ribeiro Braga, Ana Karolina Panneitz, Fernando Antônio Moreira Petri, Luís Guilherme de Oliveira

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引用次数: 0

Abstract

Circovirus porcine (PCV) is a widespread pathogen in swine, consisting of four species: PCV1, PCV2, PCV3, and PCV4. Coinfection with other pathogens exacerbates the severity of Porcine Respiratory Disease Complex (PRDC), leading to significant economic losses. In Brazil, pleurisy lesions in pigs, often due to chronic inflammation from bacterial, viral, and environmental factors, are a major economic concern. This study aimed to detect PCV2 and PCV3 in PRDC by identifying these viruses in lung and pleural samples from pigs with varying degrees of pleurisy lesions. A total of 130 lung and pleural samples were collected from a slaughterhouse and analyzed using the Slaughterhouse Pleurisy Evaluation System (SPES). The qPCR results showed that 63.9 % of lung samples tested positive for PCV2, while 29.2 % were positive for PCV3. In pleural samples, 30.7 % were positive for PCV2 and 37.7 % for PCV3. Additionally, other pathogens, including Mycoplasma hyopneumoniae (Mhyo), Actinobacillus pleuropneumoniae (APP), and Pasteurella multocida (PM), were also detected in both lung and pleural samples. Coinfections were frequently observed in both tissues. Sequencing revealed that the PCV2d variant was the most prevalent; however, we also identified positive samples for PCV2c, a rare finding in Brazil. Our study highlights the complex interactions in PRDC, as well as the presence of PCV2 and PCV3 in this context.

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猪圆环病毒（PCV2和PCV3）在不同胸膜炎评分的屠宰猪中的流行：猪肺炎支原体、胸膜肺炎放线杆菌和多杀性巴氏杆菌共感染

猪圆环病毒（PCV）是一种广泛存在于猪群中的病原体，由PCV1、PCV2、PCV3和PCV4四种病毒组成。与其他病原体的共同感染加剧了猪呼吸道疾病复合体（PRDC）的严重程度，导致重大的经济损失。在巴西，猪胸膜炎病变通常是由细菌、病毒和环境因素引起的慢性炎症引起的，这是一个主要的经济问题。本研究旨在通过在不同程度胸膜炎病变猪的肺和胸膜样本中鉴定PCV2和PCV3病毒，检测PRDC病毒。从屠宰场采集了130例肺和胸膜样本，并使用屠宰场胸膜炎评估系统（spe）进行分析。qPCR结果显示，63.9%的肺标本PCV2阳性，29.2%的肺标本PCV3阳性。在胸膜样本中，PCV2阳性占30.7%，PCV3阳性占37.7%。此外，在肺和胸膜样本中也检测到其他病原体，包括肺炎支原体（Mhyo）、胸膜肺炎放线杆菌（APP）和多杀性巴氏杆菌（PM）。在两种组织中经常观察到共感染。测序结果显示，PCV2d变异最为普遍；然而，我们也发现了PCV2c阳性样本，这在巴西很少见。我们的研究强调了PRDC中复杂的相互作用，以及在这种情况下PCV2和PCV3的存在。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Research in veterinary science 农林科学-兽医学

CiteScore

4.40

自引率

4.20%

发文量

312

审稿时长

75 days

期刊介绍： Research in Veterinary Science is an International multi-disciplinary journal publishing original articles, reviews and short communications of a high scientific and ethical standard in all aspects of veterinary and biomedical research. The primary aim of the journal is to inform veterinary and biomedical scientists of significant advances in veterinary and related research through prompt publication and dissemination. Secondly, the journal aims to provide a general multi-disciplinary forum for discussion and debate of news and issues concerning veterinary science. Thirdly, to promote the dissemination of knowledge to a broader range of professions, globally. High quality papers on all species of animals are considered, particularly those considered to be of high scientific importance and originality, and with interdisciplinary interest. The journal encourages papers providing results that have clear implications for understanding disease pathogenesis and for the development of control measures or treatments, as well as those dealing with a comparative biomedical approach, which represents a substantial improvement to animal and human health. Studies without a robust scientific hypothesis or that are preliminary, or of weak originality, as well as negative results, are not appropriate for the journal. Furthermore, observational approaches, case studies or field reports lacking an advancement in general knowledge do not fall within the scope of the journal.