The impact of pH on the size of biosynthesized silver nanoparticles: Evaluation of antioxidant and antibacterial activities

IF 5.45 Q1 Physics and Astronomy

Nano-Structures & Nano-Objects Pub Date : 2025-09-01 DOI:10.1016/j.nanoso.2025.101545

Khadija Elouardy , Mustapha Mouzaki , Hassan Ahmoum , Asmaa Akhrouf , Abdessamad Faik , Youssef Mir

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引用次数: 0

Abstract

This study reports the green synthesis of silver nanoparticles (AgNPs) using the microalga Scenedesmus sp. and their characterization via UV-Vis spectroscopy, X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, and scanning electron microscopy (SEM). AgNPs were synthesized at pH values of 4, 7, and 10 and analyzed for size and stability using dynamic light scattering (DLS). Mean particle sizes were 223 nm (pH 4), 122 nm (pH 7), and 60 nm (pH 10), with spherical morphologies observed via SEM. Zeta potential measurements indicated increased colloidal stability at higher pH. AgNPs synthesized at alkaline pH (60 nm) demonstrated enhanced antimicrobial activity against Acinetobacter baumannii ATCC196306, Escherichia coli ATCC35218, Staphylococcus aureus ATCC29213, and Pseudomonas aeruginosa ATCC27853, compared to larger nanoparticles. Similarly, antioxidant assays revealed higher activity for these smaller nanoparticles. These results suggest that pH-controlled green synthesis can produce AgNPs with optimized antimicrobial and antioxidant properties, highlighting their potential applications in biomedical fields.

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pH值对生物合成银纳米颗粒大小的影响：抗氧化和抗菌活性的评价

本研究报道了利用微藻Scenedesmus sp.绿色合成银纳米粒子（AgNPs），并通过紫外可见光谱、x射线衍射（XRD）、傅里叶变换红外光谱（FTIR）和扫描电子显微镜（SEM）对其进行了表征。在pH值为4、7和10的条件下合成AgNPs，并利用动态光散射（DLS）分析AgNPs的尺寸和稳定性。平均粒径为223 nm (pH 4), 122 nm （pH 7）和60 nm (pH 10)，通过扫描电镜观察到球形形貌。Zeta电位测量表明，在较高的pH值下，胶体稳定性增加。与较大的纳米颗粒相比，在碱性pH（60 nm）下合成的AgNPs显示出对鲍曼不动杆菌ATCC196306、大肠杆菌ATCC35218、金黄色葡萄球菌ATCC29213和铜绿假单胞菌ATCC27853的抗菌活性增强。同样，抗氧化实验显示这些小颗粒具有更高的活性。这些结果表明，ph控制绿色合成可以制备出具有最佳抗菌和抗氧化性能的AgNPs，突出了其在生物医学领域的潜在应用前景。

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来源期刊

Nano-Structures & Nano-Objects Physics and Astronomy-Condensed Matter Physics

CiteScore

9.20

自引率

0.00%

发文量

审稿时长

22 days

期刊介绍： Nano-Structures & Nano-Objects is a new journal devoted to all aspects of the synthesis and the properties of this new flourishing domain. The journal is devoted to novel architectures at the nano-level with an emphasis on new synthesis and characterization methods. The journal is focused on the objects rather than on their applications. However, the research for new applications of original nano-structures & nano-objects in various fields such as nano-electronics, energy conversion, catalysis, drug delivery and nano-medicine is also welcome. The scope of Nano-Structures & Nano-Objects involves: -Metal and alloy nanoparticles with complex nanostructures such as shape control, core-shell and dumbells -Oxide nanoparticles and nanostructures, with complex oxide/metal, oxide/surface and oxide /organic interfaces -Inorganic semi-conducting nanoparticles (quantum dots) with an emphasis on new phases, structures, shapes and complexity -Nanostructures involving molecular inorganic species such as nanoparticles of coordination compounds, molecular magnets, spin transition nanoparticles etc. or organic nano-objects, in particular for molecular electronics -Nanostructured materials such as nano-MOFs and nano-zeolites -Hetero-junctions between molecules and nano-objects, between different nano-objects & nanostructures or between nano-objects & nanostructures and surfaces -Methods of characterization specific of the nano size or adapted for the nano size such as X-ray and neutron scattering, light scattering, NMR, Raman, Plasmonics, near field microscopies, various TEM and SEM techniques, magnetic studies, etc .