Glucocorticoid Alleviates Stress-induced Hypothalamic Nerve Injury by Inhibiting the GSDMD-dependent Pyroptosis Pathway.

IF 2.7 4区医学 Q3 NEUROSCIENCES

Journal of integrative neuroscience Pub Date : 2025-08-21 DOI:10.31083/JIN39532

Shanyong Yi, Lijie Zhu, Yaxin Guo, Bin Zhao, Lai Wei, Zhijun Yao, Bin Yang

{"title":"Glucocorticoid Alleviates Stress-induced Hypothalamic Nerve Injury by Inhibiting the GSDMD-dependent Pyroptosis Pathway.","authors":"Shanyong Yi, Lijie Zhu, Yaxin Guo, Bin Zhao, Lai Wei, Zhijun Yao, Bin Yang","doi":"10.31083/JIN39532","DOIUrl":null,"url":null,"abstract":"Background: Excessive stress leads to stress injury but the underlying mechanism is not completely understood and current preventive protocols are inadequate. This study aimed to investigate if glucocorticoid (GC) reduces nerve damage in the hypothalamus caused by stress and to clarify the mechanisms involved.Methods: Behavioral alterations in stressed rats were observed using the open field test. Changes in the levels of stress hormones, inflammatory factors, and stress-related injury factors were detected using enzyme-linked immunosorbent assay (ELISA). Pathological alterations in the hypothalamus were observed using thionine staining and hematoxylin & eosin (HE) staining. The expression levels of proteins linked to pyroptosis were determined using western blotting.Results: Stressed rats presented obvious anxiety-like behavior; the levels of stress hormones, inflammatory factors, and injury-related factors fluctuated abnormally. Morphological findings indicated substantial damage in the hypothalamus. Stress-induced nerve injury was alleviated by low-dose GC treatment, which also dramatically decreased the concentrations of inflammation-associated markers and expression of the gasdermin D (GSDMD)-related pyroptosis pathway.Conclusions: Low-dose GC alleviates hypothalamic nerve injury by inhibiting the GSDMD-dependent pyroptosis pathway in stressed rats.","PeriodicalId":16160,"journal":{"name":"Journal of integrative neuroscience","volume":"24 8","pages":"39532"},"PeriodicalIF":2.7000,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of integrative neuroscience","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.31083/JIN39532","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"NEUROSCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Excessive stress leads to stress injury but the underlying mechanism is not completely understood and current preventive protocols are inadequate. This study aimed to investigate if glucocorticoid (GC) reduces nerve damage in the hypothalamus caused by stress and to clarify the mechanisms involved.

Methods: Behavioral alterations in stressed rats were observed using the open field test. Changes in the levels of stress hormones, inflammatory factors, and stress-related injury factors were detected using enzyme-linked immunosorbent assay (ELISA). Pathological alterations in the hypothalamus were observed using thionine staining and hematoxylin & eosin (HE) staining. The expression levels of proteins linked to pyroptosis were determined using western blotting.

Results: Stressed rats presented obvious anxiety-like behavior; the levels of stress hormones, inflammatory factors, and injury-related factors fluctuated abnormally. Morphological findings indicated substantial damage in the hypothalamus. Stress-induced nerve injury was alleviated by low-dose GC treatment, which also dramatically decreased the concentrations of inflammation-associated markers and expression of the gasdermin D (GSDMD)-related pyroptosis pathway.

Conclusions: Low-dose GC alleviates hypothalamic nerve injury by inhibiting the GSDMD-dependent pyroptosis pathway in stressed rats.

查看原文本刊更多论文

糖皮质激素通过抑制gsdmd依赖性焦亡通路减轻应激性下丘脑神经损伤。

背景：过度应激可导致应激性损伤，但其潜在机制尚不完全清楚，目前的预防方案也不充分。本研究旨在探讨糖皮质激素（GC）是否能减轻应激引起的下丘脑神经损伤，并阐明其机制。方法：采用开场法观察应激大鼠的行为改变。采用酶联免疫吸附试验（ELISA）检测应激激素、炎症因子和应激相关损伤因子水平的变化。采用硫氨酸染色和苏木精伊红（HE）染色观察下丘脑的病理改变。western blotting检测与焦亡相关蛋白的表达水平。结果：应激大鼠表现出明显的焦虑样行为；应激激素、炎症因子、损伤相关因子水平异常波动。形态学结果显示下丘脑有明显损伤。低剂量GC治疗可减轻应激性神经损伤，同时显著降低炎症相关标志物的浓度和气皮蛋白D （GSDMD）相关焦亡途径的表达。结论：低剂量GC通过抑制gsdmd依赖性焦亡通路减轻应激大鼠下丘脑神经损伤。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of integrative neuroscience 医学-神经科学

CiteScore

2.80

自引率

5.60%

发文量

173

审稿时长

2 months

期刊介绍： JIN is an international peer-reviewed, open access journal. JIN publishes leading-edge research at the interface of theoretical and experimental neuroscience, focusing across hierarchical levels of brain organization to better understand how diverse functions are integrated. We encourage submissions from scientists of all specialties that relate to brain functioning.