Comprehensive Analysis of Differentially Expressed Genes and Immune Infiltration in Burn Injury: Key Biomarkers and Pathways.

IF 1.8 4区医学 Q3 CRITICAL CARE MEDICINE

Journal of Burn Care & Research Pub Date : 2025-09-08 DOI:10.1093/jbcr/iraf174

Kaho Lam, Kapo Lam, Maolong Dong

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引用次数: 0

Abstract

Background: Burn injuries trigger complex immune responses and gene expression changes, impacting wound healing and systemic inflammation. Understanding these changes is crucial for identifying biomarkers and therapeutic targets.

Methods: We analyzed two GEO datasets (wound tissue (GSE8056) and blood (GSE37069)) to identify differentially expressed genes (DEGs) in burn injury samples versus controls. Immune cell proportions were assessed using CIBERSORT. Functional enrichment analyses (GO and KEGG) and protein-protein interaction (PPI) networks were constructed to identify key genes and pathways.

Results: We identified 1170 upregulated and 1227 downregulated DEGs. GO analysis revealed enrichment in neutrophil activation, inflammatory response, and extracellular matrix organization. KEGG analysis highlighted cytokine-cytokine receptor interaction, TNF, and IL-17 signaling pathways. Immune infiltration analysis showed significant changes in neutrophils, macrophages (M1/M2), and T-cell subsets. PPI network analysis identified five hub genes: JUN, STAT1, Bcl2, MMP9, and TLR2.

Conclusions: This study provides a comprehensive bioinformatic analysis of gene expression and immune responses in burn injuries. The identified DEGs, hub genes, and pathways offer insights into the immune response mechanisms and suggest potential targets for diagnostic and therapeutic interventions in burn injury management.

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烧伤损伤中差异表达基因和免疫浸润的综合分析：关键生物标志物和途径。

背景：烧伤引发复杂的免疫反应和基因表达变化，影响创面愈合和全身炎症。了解这些变化对于确定生物标志物和治疗靶点至关重要。方法：我们分析了两个GEO数据集（伤口组织（GSE8056）和血液（GSE37069）），以鉴定烧伤样本与对照组的差异表达基因（DEGs）。使用CIBERSORT评估免疫细胞比例。构建功能富集分析（GO和KEGG）和蛋白-蛋白相互作用（PPI）网络来鉴定关键基因和途径。结果：我们确定了1170个上调的deg和1227个下调的deg。氧化石墨烯分析显示中性粒细胞活化、炎症反应和细胞外基质组织富集。KEGG分析强调了细胞因子-细胞因子受体相互作用、TNF和IL-17信号通路。免疫浸润分析显示中性粒细胞、巨噬细胞（M1/M2）和t细胞亚群发生显著变化。PPI网络分析鉴定出5个枢纽基因：JUN、STAT1、Bcl2、MMP9和TLR2。结论：本研究为烧伤患者的基因表达和免疫反应提供了全面的生物信息学分析。已确定的deg、中心基因和途径为了解免疫反应机制提供了见解，并为烧伤管理的诊断和治疗干预提供了潜在的目标。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Burn Care & Research 医学-外科

CiteScore

2.60

自引率

21.40%

发文量

535

审稿时长

4-8 weeks

期刊介绍： Journal of Burn Care & Research provides the latest information on advances in burn prevention, research, education, delivery of acute care, and research to all members of the burn care team. As the official publication of the American Burn Association, this is the only U.S. journal devoted exclusively to the treatment and research of patients with burns. Original, peer-reviewed articles present the latest information on surgical procedures, acute care, reconstruction, burn prevention, and research and education. Other topics include physical therapy/occupational therapy, nutrition, current events in the evolving healthcare debate, and reports on the newest computer software for diagnostics and treatment. The Journal serves all burn care specialists, from physicians, nurses, and physical and occupational therapists to psychologists, counselors, and researchers.