Point application therapy targeting Feishu, Pishu and Shenshu attenuates asthma by suppressing autophagy and the AMPK-mTOR signalling pathway.

IF 1.3 4区 医学 Q3 ALLERGY
Yutong Jin, Yena Gu, Lihua Xuan
{"title":"Point application therapy targeting Feishu, Pishu and Shenshu attenuates asthma by suppressing autophagy and the AMPK-mTOR signalling pathway.","authors":"Yutong Jin, Yena Gu, Lihua Xuan","doi":"10.1080/02770903.2025.2558759","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>In traditional Chinese medicine, asthma is associated with deficiencies in Lung Qi, Spleen Qi, and Kidney Qi. This study investigated the therapeutic mechanism of point application therapy focusing on the acupoints Feishu (BL13), Pishu (BL20), and Shenshu (BL23) for asthma treatment.</p><p><strong>Methods: </strong>An asthma model was established in Wistar rats <i>via</i> intraperitoneal ovalbumin injection combined with nebulization. Therapeutic ointments were prepared using white mustard seed, Yanhusuo, Gansui and Asarum sieboldii, which were applied topically to the Feishu (BL13), Pishu (BL20) and Shenshu (BL23) acupoints. To examine the mechanistic basis, the AMPK inhibitor dorsomorphin (compound C, CC) was employed. We subsequently measured inflammatory factor levels in rat serum and bronchoalveolar lavage fluid, quantified AMPK/mTOR pathway-related protein expression, and assessed pulmonary inflammation alongside collagen deposition in airway mucosa.</p><p><strong>Results: </strong>The point application therapy significantly ameliorated asthma symptoms and reduced levels of inflammatory factors, including interleukin (IL)-6 and IL-1<i>β</i>, in both serum and bronchoalveolar lavage fluid. The treatment additionally decreased expression levels of mTOR, LC3, NF-κB and TGF-<i>β</i>1 while increasing AMPK content. Histological examination revealed reduced inflammatory cell infiltration and diminished collagen deposition in both lung and airway tissues. Crucially, administration of the AMPK inhibitor dorsomorphin substantially attenuated the therapeutic efficacy of point application therapy in asthmatic rats.</p><p><strong>Conclusions: </strong>Point application therapy targeting Feishu, Pishu and Shenshu acupoints inhibits autophagy in airway and lung tissues in asthma. These therapeutic effects are mediated, at least partially, through the AMPK/mTOR pathway.</p>","PeriodicalId":15076,"journal":{"name":"Journal of Asthma","volume":" ","pages":"1-11"},"PeriodicalIF":1.3000,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Asthma","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/02770903.2025.2558759","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ALLERGY","Score":null,"Total":0}
引用次数: 0

Abstract

Objective: In traditional Chinese medicine, asthma is associated with deficiencies in Lung Qi, Spleen Qi, and Kidney Qi. This study investigated the therapeutic mechanism of point application therapy focusing on the acupoints Feishu (BL13), Pishu (BL20), and Shenshu (BL23) for asthma treatment.

Methods: An asthma model was established in Wistar rats via intraperitoneal ovalbumin injection combined with nebulization. Therapeutic ointments were prepared using white mustard seed, Yanhusuo, Gansui and Asarum sieboldii, which were applied topically to the Feishu (BL13), Pishu (BL20) and Shenshu (BL23) acupoints. To examine the mechanistic basis, the AMPK inhibitor dorsomorphin (compound C, CC) was employed. We subsequently measured inflammatory factor levels in rat serum and bronchoalveolar lavage fluid, quantified AMPK/mTOR pathway-related protein expression, and assessed pulmonary inflammation alongside collagen deposition in airway mucosa.

Results: The point application therapy significantly ameliorated asthma symptoms and reduced levels of inflammatory factors, including interleukin (IL)-6 and IL-1β, in both serum and bronchoalveolar lavage fluid. The treatment additionally decreased expression levels of mTOR, LC3, NF-κB and TGF-β1 while increasing AMPK content. Histological examination revealed reduced inflammatory cell infiltration and diminished collagen deposition in both lung and airway tissues. Crucially, administration of the AMPK inhibitor dorsomorphin substantially attenuated the therapeutic efficacy of point application therapy in asthmatic rats.

Conclusions: Point application therapy targeting Feishu, Pishu and Shenshu acupoints inhibits autophagy in airway and lung tissues in asthma. These therapeutic effects are mediated, at least partially, through the AMPK/mTOR pathway.

穴位贴敷治疗肺俞、脾俞、肾俞通过抑制自噬和AMPK-mTOR信号通路减轻哮喘。
目的:中医认为哮喘与肺气、脾气、肾气不足有关。本研究以肺俞(BL13)、脾俞(BL20)、肾俞(BL23)为穴位,探讨穴位贴敷法治疗哮喘的作用机制。方法:腹腔注射卵清蛋白联合雾化,建立Wistar大鼠哮喘模型。采用白芥子、炎虎索、甘穗、三叶草细辛配制治疗软膏,分别外敷肺俞(BL13)、脾俞(BL20)、肾俞(BL23)穴位。为了研究其机制基础,我们使用了AMPK抑制剂dorsomorphin(化合物C, CC)。随后,我们测量了大鼠血清和支气管肺泡灌洗液中的炎症因子水平,量化了AMPK/mTOR通路相关蛋白的表达,并评估了肺部炎症与气道粘膜胶原沉积的关系。结果:穴位贴敷治疗可显著改善哮喘症状,降低血清和支气管肺泡灌洗液中炎症因子,包括白细胞介素(IL)-6和IL-1β水平。此外,处理降低mTOR、LC3、NF-κB和TGF-β1的表达水平,增加AMPK含量。组织学检查显示肺和气道组织炎症细胞浸润减少,胶原沉积减少。至关重要的是,使用AMPK抑制剂dorsomorphin大大降低了穴位贴敷治疗哮喘大鼠的疗效。结论:穴位贴敷治疗哮喘患者肺俞、脾俞、肾俞穴可抑制气道及肺组织自噬。这些治疗效果至少部分通过AMPK/mTOR通路介导。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Journal of Asthma
Journal of Asthma 医学-过敏
CiteScore
4.00
自引率
5.30%
发文量
158
审稿时长
3-8 weeks
期刊介绍: Providing an authoritative open forum on asthma and related conditions, Journal of Asthma publishes clinical research around such topics as asthma management, critical and long-term care, preventative measures, environmental counselling, and patient education.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信