Whole-genome sequencing and comparative genome analysis of three multidrug-resistant clinical uropathogenic Escherichia coli isolates from India.

IF 3.2 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of Applied Microbiology Pub Date : 2025-09-01 DOI:10.1093/jambio/lxaf228

Atanu Manna, Ramya Ramadoss, Vanathy K, Srirangaraj S, Ramya R, Dhamodharan Ramasamy, Divya Lakshmanan

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引用次数: 0

Abstract

Aim: To investigate the phenotypic and genomic features of three multidrug-resistant clinical mucoid and non-mucoid uropathogenic Escherichia coli (UPEC) strains to understand their antimicrobial resistance, biofilm formation, and virulence in urinary tract infections (UTIs).

Methods and results: The UPEC strains A5, A10, and A15 were isolated from two UTI patients. Phenotypic assays included colony morphology, antibiotic susceptibility, motility, and biofilm formation. Whole genome sequencing identified resistance genes, virulence factors, sequence types, serotypes, plasmid types, and gene variations. While strains A5 and A10 were mucoid, A15 was non-mucoid. Although obtained from a single patient, A10 formed biofilm while A5 did not. Both strains harboured blaNDM-5 and blaCMY-145, while A15 carried blaCTX-M-15. Genomic variations in A10 included mutations in fdeC and chiA (biofilm genes), while A15 showed changes in fyuA, pdeR, pdeF, and waaZ. A comparative genome analysis of our strains with Indian UPEC isolates revealed that the presence of blaCMY-145 in strains A5 and A10 may be rare.

Conclusions: Despite the genetic similarity, UPEC strains exhibit distinct phenotypes possibly due to variations in inducible conditions, including host and environmental factors. These differences highlight the multifactorial nature of UPEC pathogenicity and the influence of both genetic and host-related factors.

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印度三株临床尿路致病性多药耐药大肠杆菌的全基因组测序和比较基因组分析。

目的：研究3株临床多药耐药（MDR）黏液样和非黏液样尿路致病性大肠埃希菌（UPEC）的表型和基因组特征，了解其在尿路感染（uti）中的耐药性、生物膜形成和毒力。方法与结果：从2例尿路感染患者中分离到UPEC菌株A5、A10和A15。表型分析包括菌落形态、抗生素敏感性、运动性和生物膜形成。全基因组测序（WGS）鉴定了抗性基因、毒力因子、序列型（ST）、血清型、质粒型和基因变异。菌株A5和A10为黏液样，菌株A15为非黏液样。虽然来自单个患者，但A10形成了生物膜，而A5没有。这两个菌株都携带blaNDM-5和blaCMY-145，而A15携带blaCTX-M-15。A10的基因组变异包括fdeC和chiA（生物膜基因）的突变，而A15则显示fyuA、pdeR、pdeF和waaZ的变化。我们的菌株与印度UPEC分离株的比较基因组分析显示，菌株A5和A10中blaCMY-145的存在可能是罕见的。结论：尽管遗传相似，但UPEC菌株表现出不同的表型，可能是由于诱导条件的差异，包括宿主和环境因素。这些差异突出了upc致病性的多因素性质以及遗传和宿主相关因素的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Applied Microbiology 生物-生物工程与应用微生物

CiteScore

7.30

自引率

2.50%

发文量

427

审稿时长

2.7 months

期刊介绍： Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.