The protection of sulforaphane on subarachnoid hemorrhage-induced intestinal mucosa injury in rats.

IF 3.9 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Frontiers in Molecular Biosciences Pub Date : 2025-08-22 eCollection Date: 2025-01-01 DOI:10.3389/fmolb.2025.1635795

Zixiang Liu, Pengpeng Li, Yuanhai Zhang, Shidi Zhao, Wei Gao

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引用次数: 0

Abstract

Introduction: Sulforaphane (SFN) is recognized for its anti-inflammatory properties; however, the underlying molecular mechanisms remain unclear. In this study, we explored the effect of SFN on subarachnoid hemorrhage (SAH) and the potential mechanisms.

Methods: Sprague-Dawley (SD) rats were divided into three groups (n = 12): Sham + vehicle group (Sham + V), SAH + vehicle group (SAH + V), and SAH + SFN group (SAH + S). SFN (50 mg/kg) dissolved in 250-280 μL corn oil was intraperitoneally injected, and the same volume of corn oil was served as the control. The appetite score, gut wet/dry weight ratio, and histological changes in ileum tissues were examined to determine intestinal mucosal injury. Quantitative real-time PCR (qRT-PCR) and Western blot were performed to examine the expression of genes. LC3 immunofluorescence and Hoechst 33258 staining were used to assess cell autophagy and apoptosis.

Results: Compared to the SAH + V group, the SAH + S group demonstrated a significantly increased appetite score (1.55 ± 0.23 vs. 1.90 ± 0.35); decreased gut wet/dry weight ratio (4.02 ± 0.21 vs. 3.18 ± 0.21) and inflammatory score (2.89 ± 0.33 vs. 1.89 ± 0.60); elevated mRNA expression of Nrf-2 (1.12 ± 0.14 vs. 1.89 ± 0.12), HO-1 (0.46 ± 0.02 vs. 1.02 ± 0.10), and NQO-1 (1.35 ± 0.09 vs. 1.97 ± 0.18); and elevated protein levels of Nrf-2 (0.92 ± 0.18 vs. 1.43 ± 0.23), Keap1 (0.31 ± 0.03 vs. 0.44 ± 0.02), HO-1 (0.65 ± 0.02 vs. 0.88 ± 0.02), NQO-1 (0.58 ± 0.02 vs. 0.78 ± 0.02), LC3-II/I (0.20 ± 0.004 vs. 0.28 ± 0.01), ATG4D (0.45 ± 0.01 vs. 0.72 ± 0.04), and P62 (0.85 ± 0.01 vs. 0.99 ± 0.03). The in vitro experiments further revealed that 3-methyladenine (3-MA) significantly reversed the decreased apoptosis of IEC-6 cells induced by 20 μmol/L SFN (20.60 ± 1.28 vs. 11.50 ± 0.58).

Conclusion: SFN exhibited the protective effect on intestinal mucosa injury after SAH via activating autophagy, which may provide an innovative approach to alleviate the intestinal mucosa injury caused by SAH.

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萝卜硫素对大鼠蛛网膜下腔出血性肠黏膜损伤的保护作用。

简介：萝卜硫素（SFN）因其抗炎特性而被公认；然而，潜在的分子机制尚不清楚。在本研究中，我们探讨了SFN对蛛网膜下腔出血（SAH）的影响及其可能的机制。方法：将SD大鼠分为3组（n = 12）： Sham + vehicle组（Sham + V）、SAH + vehicle组（SAH + V）、SAH + SFN组（SAH + S）。腹腔注射溶于250 ~ 280 μL玉米油中的SFN (50 mg/kg)，并以相同体积的玉米油为对照。检查食欲评分、肠道干/湿重比和回肠组织组织学变化，以确定肠黏膜损伤。采用实时荧光定量PCR （qRT-PCR）和Western blot检测基因表达情况。LC3免疫荧光和Hoechst 33258染色检测细胞自噬和凋亡。结果：与SAH + V组相比，SAH + S组食欲评分显著升高（1.55±0.23∶1.90±0.35）；降低肠道干/湿重比（4.02±0.21比3.18±0.21）和炎症评分（2.89±0.33比1.89±0.60）；Nrf-2（1.12±0.14比1.89±0.12）、HO-1（0.46±0.02比1.02±0.10）、nqos -1(1.35±0.09比1.97±0.18)mRNA表达升高；Nrf-2（0.92±0.18比1.43±0.23）、Keap1（0.31±0.03比0.44±0.02）、HO-1（0.65±0.02比0.88±0.02）、NQO-1（0.58±0.02比0.78±0.02）、LC3-II/I（0.20±0.004比0.28±0.01）、ATG4D（0.45±0.01比0.72±0.04）、P62（0.85±0.01比0.99±0.03）蛋白水平升高。体外实验进一步表明，3-甲基腺嘌呤（3-MA）显著逆转20 μmol/L SFN诱导的IEC-6细胞凋亡减少（20.60±1.28∶11.50±0.58）。结论：SFN通过激活自噬对SAH后肠黏膜损伤具有保护作用，可能为减轻SAH后肠黏膜损伤提供一种创新途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Molecular Biosciences Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

7.20

自引率

4.00%

发文量

1361

审稿时长

14 weeks

期刊介绍： Much of contemporary investigation in the life sciences is devoted to the molecular-scale understanding of the relationships between genes and the environment — in particular, dynamic alterations in the levels, modifications, and interactions of cellular effectors, including proteins. Frontiers in Molecular Biosciences offers an international publication platform for basic as well as applied research; we encourage contributions spanning both established and emerging areas of biology. To this end, the journal draws from empirical disciplines such as structural biology, enzymology, biochemistry, and biophysics, capitalizing as well on the technological advancements that have enabled metabolomics and proteomics measurements in massively parallel throughput, and the development of robust and innovative computational biology strategies. We also recognize influences from medicine and technology, welcoming studies in molecular genetics, molecular diagnostics and therapeutics, and nanotechnology. Our ultimate objective is the comprehensive illustration of the molecular mechanisms regulating proteins, nucleic acids, carbohydrates, lipids, and small metabolites in organisms across all branches of life. In addition to interesting new findings, techniques, and applications, Frontiers in Molecular Biosciences will consider new testable hypotheses to inspire different perspectives and stimulate scientific dialogue. The integration of in silico, in vitro, and in vivo approaches will benefit endeavors across all domains of the life sciences.