Study of Bacteriostasis of Kaempferide on Foodborne Pathogenic Bacteria by Indirect Determination of Capillary Electrophoresis.

IF 2.5 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

ELECTROPHORESIS Pub Date : 2025-09-08 DOI:10.1002/elps.70023

Qingqing Wang, Junlan Ye, Wei Wang

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Abstract

Foodborne pathogenic bacteria always threaten human health. Flavonoids are commonly used in antibacterial applications. Studying the antibacterial effect of flavonoids on bacteria is significant. Capillary electrophoresis is a versatile separation means. It has different separation modes, so, it can be applied in the analysis of both bacteria and flavonoids. In this study, an efficient method of indirect separation and detection of three bacteria Vibrio parahaemolyticus, Escherichia coli, and Staphylococcus aureus was developed. The determination of bacteria was realized by separating the aptamers corresponding to the bacteria by capillary sieving electrophoresis. This method avoided the disadvantages of low resolution, channel adsorption and bacterial aggregation, encountered in the direct separation of bacteria. The separation of the flavonoid kaempferide was realized by capillary zone electrophoresis, and kaempferide in Kaempferia galanga was determined. Kaempferide and the extract of Kaempferia galanga were applied in the antibacterial tests separately. Using the developed method, the bacterial growth curves and the curves of the kaempferide concentration were studied respectively. The results showed that both kaempferide and Kaempferia galanga could effectively inhibit the bacteria. The inhibitory effect on the terrestrial bacteria Escherichia coli, and Staphylococcus aureus was better than that on the marine bacterium Vibrio parahaemolyticus.

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间接毛细管电泳法研究山奈啶对食源性致病菌的抑菌作用。

食源性致病菌一直威胁着人类的健康。黄酮类化合物通常用于抗菌应用。研究黄酮类化合物对细菌的抑菌作用具有重要意义。毛细管电泳是一种用途广泛的分离手段。它具有不同的分离方式，因此既可用于细菌的分析，也可用于类黄酮的分析。本研究建立了副溶血性弧菌、大肠杆菌和金黄色葡萄球菌三种细菌的间接分离检测方法。通过毛细管筛分电泳分离细菌对应的适体，实现细菌的测定。该方法避免了直接分离细菌所遇到的分辨率低、通道吸附和细菌聚集等缺点。采用毛细管区带电泳法分离山奈黄酮，测定了山奈黄酮在高良慈母中的含量。采用山奈素和山奈良姜提取物分别进行抑菌试验。用该方法分别研究了细菌生长曲线和山奈啶浓度曲线。结果表明，山奈素和山奈良姜均能有效抑制病原菌。对陆生细菌大肠杆菌、金黄色葡萄球菌的抑制效果优于对海洋细菌副溶血性弧菌的抑制效果。

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来源期刊

ELECTROPHORESIS 生物-分析化学

CiteScore

6.30

自引率

13.80%

发文量

244

审稿时长

1.9 months

期刊介绍： ELECTROPHORESIS is an international journal that publishes original manuscripts on all aspects of electrophoresis, and liquid phase separations (e.g., HPLC, micro- and nano-LC, UHPLC, micro- and nano-fluidics, liquid-phase micro-extractions, etc.). Topics include new or improved analytical and preparative methods, sample preparation, development of theory, and innovative applications of electrophoretic and liquid phase separations methods in the study of nucleic acids, proteins, carbohydrates natural products, pharmaceuticals, food analysis, environmental species and other compounds of importance to the life sciences. Papers in the areas of microfluidics and proteomics, which are not limited to electrophoresis-based methods, will also be accepted for publication. Contributions focused on hyphenated and omics techniques are also of interest. Proteomics is within the scope, if related to its fundamentals and new technical approaches. Proteomics applications are only considered in particular cases. Papers describing the application of standard electrophoretic methods will not be considered. Papers on nanoanalysis intended for publication in ELECTROPHORESIS should focus on one or more of the following topics: • Nanoscale electrokinetics and phenomena related to electric double layer and/or confinement in nano-sized geometry • Single cell and subcellular analysis • Nanosensors and ultrasensitive detection aspects (e.g., involving quantum dots, "nanoelectrodes" or nanospray MS) • Nanoscale/nanopore DNA sequencing (next generation sequencing) • Micro- and nanoscale sample preparation • Nanoparticles and cells analyses by dielectrophoresis • Separation-based analysis using nanoparticles, nanotubes and nanowires.