A Low-Voltage-Driven Droplet Sorter for High-Stability and Small-Deformation Droplet Sorting.

IF 2.5 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

ELECTROPHORESIS Pub Date : 2025-09-08 DOI:10.1002/elps.70025

Lin Jiang, Qingqing Liu, Hang Yang, Qinhong Wang, Nan Xiang

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引用次数: 0

Abstract

Electric droplet sorting is widely applied in the screening of target molecules, cells, drugs, and microparticles. Previous studies have made several optimizations on the electrode materials, structures, and arrangements. However, voltages of over 1 kV are required to realize droplet sorting, which causes the undesired droplet splitting. In addition, great droplet deformation caused by the high voltage decreases the sorting accuracy and generates negative effects on the targets encapsulated within the droplets. In this study, we developed a low-voltage-driven droplet sorter (LV-DS) for droplet sorting with high stability and small deformation. Our LV-DS consisted of one square active and four triangular ground electrodes which were fabricated using liquid metal with higher stability and higher electrical conductivity than the previous electrode materials of low-melting alloy and salt water. Our LV-DS could realize the high-stability droplet sorting with small deformation at the voltage as low as 80 V. The results showed that our LV-DS presented great prospects in the high-stability droplet sorting with a low voltage. In addition, the advantage of small droplet deformation makes our LV-DS be attractive for the applications, such as droplet-based cell sorting with a high viability and droplet-based material screening with a small deformation.

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一种用于高稳定性和小变形液滴分选的低压驱动液滴分选机。

电液滴分选广泛应用于靶分子、细胞、药物、微粒的筛选。以往的研究对电极的材料、结构和排列进行了多次优化。但要实现液滴分选，需要超过1 kV的电压，这会导致液滴分裂。此外，高压引起的大液滴变形降低了分选精度，并对液滴内包裹的目标产生负面影响。在这项研究中，我们开发了一种低压驱动的液滴分选机（LV-DS），它具有高稳定性和小变形。我们的LV-DS由1个方形有源电极和4个三角形地电极组成，它们是用液态金属制成的，比以前的低熔合金和盐水电极材料具有更高的稳定性和导电性。在低至80 V的电压下，我们的LV-DS可以实现高稳定性、小变形的液滴分选。结果表明，LV-DS在低电压、高稳定性的液滴分选中具有广阔的应用前景。此外，液滴变形小的优势使我们的LV-DS具有很高的应用吸引力，例如具有高活力的基于液滴的细胞分选和具有小变形的基于液滴的材料筛选。

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来源期刊

ELECTROPHORESIS 生物-分析化学

CiteScore

6.30

自引率

13.80%

发文量

244

审稿时长

1.9 months

期刊介绍： ELECTROPHORESIS is an international journal that publishes original manuscripts on all aspects of electrophoresis, and liquid phase separations (e.g., HPLC, micro- and nano-LC, UHPLC, micro- and nano-fluidics, liquid-phase micro-extractions, etc.). Topics include new or improved analytical and preparative methods, sample preparation, development of theory, and innovative applications of electrophoretic and liquid phase separations methods in the study of nucleic acids, proteins, carbohydrates natural products, pharmaceuticals, food analysis, environmental species and other compounds of importance to the life sciences. Papers in the areas of microfluidics and proteomics, which are not limited to electrophoresis-based methods, will also be accepted for publication. Contributions focused on hyphenated and omics techniques are also of interest. Proteomics is within the scope, if related to its fundamentals and new technical approaches. Proteomics applications are only considered in particular cases. Papers describing the application of standard electrophoretic methods will not be considered. Papers on nanoanalysis intended for publication in ELECTROPHORESIS should focus on one or more of the following topics: • Nanoscale electrokinetics and phenomena related to electric double layer and/or confinement in nano-sized geometry • Single cell and subcellular analysis • Nanosensors and ultrasensitive detection aspects (e.g., involving quantum dots, "nanoelectrodes" or nanospray MS) • Nanoscale/nanopore DNA sequencing (next generation sequencing) • Micro- and nanoscale sample preparation • Nanoparticles and cells analyses by dielectrophoresis • Separation-based analysis using nanoparticles, nanotubes and nanowires.