Electroacupuncture Improves Myocardial Fibrosis in Association With Intestinal Flora and Metabolic Modulation in Mice.

IF 2.1 4区医学 Q3 CARDIAC & CARDIOVASCULAR SYSTEMS

Cardiology Research and Practice Pub Date : 2025-08-28 eCollection Date: 2025-01-01 DOI:10.1155/crp/7765150

Xueyun Que, Feiyu He, Qida He, Jieying Lim, Xiaoshuang Li, Xianjun Meng, Zongbao Yang

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引用次数: 0

Abstract

Objective: This study investigates the reparative effect of electroacupuncture on myocardial fibrosis (MF) in mice and explores its impact on intestinal flora and metabolism profile. This examines an investigation into the biological mechanisms underlying electroacupuncture's efficacy in treating MF in mice. Methods: Twenty-four male Kunming mice (27-34 g) were randomized into three groups: normal control (NC, n = 8), MF model (MF, n = 8), and electroacupuncture treatment (EA, n = 8). MF and EA groups received daily subcutaneous ISO injections (25 mg/kg) at the nape for 5 days; NC mice received saline. The EA group underwent 14 days of EA at PC6 (Neiguan). Cardiac function, intestinal flora, and metabolites were assessed post-treatment. Results: EA significantly reduced cardiac weight index (CWI), collagen volume fraction (CVF), and serum procollagen III N-terminal propeptide (PIIINP) and improved left ventricular ejection fraction (LVEF) and shortening fraction (LVFS) (p < 0.05). Gut microbiota analysis revealed distinct composition shifts: EA restored Bacteroidota abundance and lowered Firmicutes/Bacteroidota ratios, resembling NC profiles. Notably, differential bacteria (e.g., Staphylococcus lentus, Xylanophilum) correlated with PIIINP, CVF, and cardiac function. Metabolomics identified reduced TMAO, phenylalanine, acetone, and lactic acid in EA vs. MF (p < 0.05). Negative correlations included Stricto-1 vs. phenylalanine and Rodentium vs. acetone. Conclusion: EA ameliorates ISO-induced MF in mice by modulating gut microbiota structure and metabolic profiles, suggesting a microbiota-metabolite axis mediates its therapeutic effects.

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电针改善小鼠心肌纤维化与肠道菌群和代谢调节的关系。

目的：研究电针对小鼠心肌纤维化（MF）的修复作用，并探讨其对肠道菌群和代谢谱的影响。本研究探讨了电针治疗小鼠MF的生物学机制。方法：24只雄性昆明小鼠（27 ~ 34 g）随机分为正常对照组（NC, n = 8）、MF模型组（MF, n = 8）和电针治疗组（EA, n = 8）。MF组和EA组每日颈背皮下注射ISO (25 mg/kg)，连续5 d；NC小鼠接受生理盐水。EA组于PC6（内关）进行14天的EA治疗。治疗后评估心功能、肠道菌群和代谢物。结果：EA显著降低心脏重量指数（CWI）、胶原体积分数（CVF）、血清III型前胶原n端前肽（PIIINP），提高左心室射血分数（LVEF）和缩短分数（LVFS）（p < 0.05）。肠道菌群分析显示出明显的组成变化：EA恢复了拟杆菌门的丰度，降低了厚壁菌门/拟杆菌门的比例，与NC相似。值得注意的是，不同的细菌（如：葡萄球菌、嗜木杆菌）与PIIINP、CVF和心功能相关。代谢组学发现，EA与MF相比，TMAO、苯丙氨酸、丙酮和乳酸含量降低（p < 0.05）。负相关包括Stricto-1与苯丙氨酸和Rodentium与丙酮。结论：EA通过调节肠道菌群结构和代谢谱改善iso诱导的小鼠MF，提示其治疗作用可能与菌群代谢轴有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cardiology Research and Practice Medicine-Cardiology and Cardiovascular Medicine

CiteScore

4.40

自引率

0.00%

发文量

审稿时长

13 weeks

期刊介绍： Cardiology Research and Practice is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies that focus on the diagnosis and treatment of cardiovascular disease. The journal welcomes submissions related to systemic hypertension, arrhythmia, congestive heart failure, valvular heart disease, vascular disease, congenital heart disease, and cardiomyopathy.