Mathieu Victor Paulin, Thomas Schermerhorn, Dorsa Mehrabanpour, Suraj Unniappan, Elisabeth Snead
{"title":"Plasma Arginine Vasopressin and Serum Copeptin Concentrations at Rest and After Osmotic- and Non-Osmotic-Based Stimulation Tests in Dogs","authors":"Mathieu Victor Paulin, Thomas Schermerhorn, Dorsa Mehrabanpour, Suraj Unniappan, Elisabeth Snead","doi":"10.1111/jvim.70219","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>Serum copeptin (sCoP) is used as a surrogate for plasma arginine vasopressin (pAVP) measurement in humans.</p>\n </section>\n \n <section>\n \n <h3> Objective</h3>\n \n <p>To measure pAVP and sCoP at rest and after osmotic- and non-osmotic stimulation testing in dogs.</p>\n </section>\n \n <section>\n \n <h3> Animals</h3>\n \n <p>Eight young castrated/spayed healthy research Beagles, eight young intact dogs, and eight old neutered healthy client-owned dogs.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>In this prospective longitudinal study, pAVP and sCoP were measured under iso-(baseline), hypo-(water load followed by intravenous administration of desmopressin [WLT]), and hyper-(water deprivation test [WDT]) osmolar conditions assessed by measured plasma osmolality (pOsm<sub>(m)</sub>), and after administration of arginine (AST), IV, and Bovril (BST), PO. The fraction of change (<i>F</i>) in a variable <i>y</i> (e.g., pAVP) between baseline (<i>T</i><sub>0</sub>) and a timepoint <i>X</i> (<i>T</i><sub><i>X</i></sub>) during testing was defined as <i>Fy</i> = [<i>y</i> (<i>T</i><sub><i>X</i></sub>) – <i>y</i> (<i>T</i><sub>0</sub>)]/<i>y</i> (<i>T</i><sub>0</sub>).</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Baseline sCoP had wide inter-individual variations. Mean [range] FpAVP and FsCoP at the end of WDT were +110% [+80; +142] and +18% [+0.4; +38] compared to baseline, respectively. Mean [range] FpAVP after water load and FsCoP after water load followed by desmopressin administration were −22% [−48; −0.5] and −29% [−39; −14] compared to baseline, respectively. Both FpAVP and FsCoP were strongly correlated to FpOsm<sub>(m)</sub> (<i>r</i> = +0.76, <i>p</i> = 0.004; <i>r</i> = +0.78, <i>p</i> = 0.002; respectively). When sCoP was measured at <i>T</i><sub>4h</sub> instead of <i>T</i><sub>2h</sub> during WLT, to reflect its longer half-life reported in humans, the correlation between FpAVP<sub>P800</sub> and FsCoP became excellent (<i>r</i> = +0.90, <i>p</i> < 0.001). No stimulation of sCoP secretion occurred during AST or BST.</p>\n </section>\n \n <section>\n \n <h3> Conclusion and Clinical Importance</h3>\n \n <p>Serum CoP could be used as a surrogate for pAVP measurement in healthy dogs.</p>\n </section>\n </div>","PeriodicalId":49958,"journal":{"name":"Journal of Veterinary Internal Medicine","volume":"39 5","pages":""},"PeriodicalIF":2.2000,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jvim.70219","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Veterinary Internal Medicine","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jvim.70219","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Background
Serum copeptin (sCoP) is used as a surrogate for plasma arginine vasopressin (pAVP) measurement in humans.
Objective
To measure pAVP and sCoP at rest and after osmotic- and non-osmotic stimulation testing in dogs.
Animals
Eight young castrated/spayed healthy research Beagles, eight young intact dogs, and eight old neutered healthy client-owned dogs.
Methods
In this prospective longitudinal study, pAVP and sCoP were measured under iso-(baseline), hypo-(water load followed by intravenous administration of desmopressin [WLT]), and hyper-(water deprivation test [WDT]) osmolar conditions assessed by measured plasma osmolality (pOsm(m)), and after administration of arginine (AST), IV, and Bovril (BST), PO. The fraction of change (F) in a variable y (e.g., pAVP) between baseline (T0) and a timepoint X (TX) during testing was defined as Fy = [y (TX) – y (T0)]/y (T0).
Results
Baseline sCoP had wide inter-individual variations. Mean [range] FpAVP and FsCoP at the end of WDT were +110% [+80; +142] and +18% [+0.4; +38] compared to baseline, respectively. Mean [range] FpAVP after water load and FsCoP after water load followed by desmopressin administration were −22% [−48; −0.5] and −29% [−39; −14] compared to baseline, respectively. Both FpAVP and FsCoP were strongly correlated to FpOsm(m) (r = +0.76, p = 0.004; r = +0.78, p = 0.002; respectively). When sCoP was measured at T4h instead of T2h during WLT, to reflect its longer half-life reported in humans, the correlation between FpAVPP800 and FsCoP became excellent (r = +0.90, p < 0.001). No stimulation of sCoP secretion occurred during AST or BST.
Conclusion and Clinical Importance
Serum CoP could be used as a surrogate for pAVP measurement in healthy dogs.
期刊介绍：
The mission of the Journal of Veterinary Internal Medicine is to advance veterinary medical knowledge and improve the lives of animals by publication of authoritative scientific articles of animal diseases.
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