A highly active self-assembled nanozyme based on cascade system for dual-mode detection of sarcosine

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2025-09-08 DOI:10.1007/s00604-025-07501-6

Yanke Zhai, Dan Wang, Qianqing Lin, Ruixin Yang, Guo Chen, Xiaotong Li, Haonan Jin, Hualu Zhang, Yibing Ji, Ruijun Li

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Abstract

A nanozyme-mediated cascade reaction system for fluorometric and colorimetric dual-mode detection of sarcosine (SA) was developed. The nanozymes (Zn-Glu@Hemin) were synthesized via a rapid self-assembly within 10 min at room temperature. Importantly, the Zn-Glu@Hemin exhibited strong peroxidase (POD)-mimicking activity, catalyzing the generation of hydroxyl radical (·OH) and superoxide anion (O₂^·−) from hydrogen peroxide (H₂O₂), enhancing the fluorescence reaction of o-phenylenediamine (OPD) and the colorimetric reaction of 3,3′,5,5′-tetramethylbenzidine (TMB). Thus, a dual-mode sensing platform is created by combining SA and sarcosine oxidase (SOD) into the enzyme cascade system (SA-SOD-POD). The developed sensors exhibited superior performance, with high sensitivity and selectivity for SA detection. The detection limits were 0.14 μM (fluorescence) and 0.26 μM (colorimetric), with detection ranges of 1–120 μM and 1–70 μM, respectively. The system was incorporated into both a smartphone-assisted dual-signal sensing platform and a portable field detection kit, demonstrating practical applicability. Besides, the practicability of the established approach was confirmed in human serum samples with reliable recoveries. This work shows advantages in the synthesis method and detection sensitivity of nanozymes, which can accurately detect disease biomarkers and show high potential in real-time diagnosis.

Graphical Abstract

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基于级联系统的高活性自组装纳米酶用于肌氨酸双模检测

建立了一种纳米酶介导的级联反应体系，用于荧光和比色双模检测肌氨酸（SA）。纳米酶（Zn-Glu@Hemin）在室温下通过快速自组装在10分钟内合成。重要的是，Zn-Glu@Hemin具有较强的过氧化物酶（POD）模拟活性，催化过氧化氢（H2O2）生成羟基自由基（·OH）和超氧阴离子（O2·−），增强邻苯二胺（OPD）的荧光反应和3,3 ',5,5 ' -四甲基联苯胺（TMB）的比色反应。因此，将SA和SOD结合成酶级联系统（SA-SOD- pod），形成双模传感平台。所研制的传感器性能优越，对SA检测具有较高的灵敏度和选择性。检测限分别为0.14 μM（荧光）和0.26 μM（比色），检测范围分别为1 ~ 120 μM和1 ~ 70 μM。该系统被集成到智能手机辅助双信号传感平台和便携式现场检测套件中，展示了实用性。此外，建立的方法在人血清样品中具有可靠的回收率。本工作显示了纳米酶在合成方法和检测灵敏度上的优势，可以准确检测疾病生物标志物，在实时诊断方面具有很高的潜力。图形抽象

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.