Dual-mode nanozyme-hydrogel platform for on-site multi-target detection of biomarkers and hazardous substances

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2025-09-08 DOI:10.1007/s00604-025-07516-z

Junjie Zhao, Jiawei Hong, Haonan Li, Haonan Zhang, Longshan Zhao

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引用次数: 0

Abstract

A novel dual-mode sensing system integrating a magnetic core–shell CuFe₂O₄/Cu/MnO₂ nanozyme with a stimuli-responsive agarose-deep eutectic solvent hydrogel (DES-Aga) is reported. The nanozyme exhibits exceptional oxidase-like activity, characterized by a low Michaelis constant (K_m = 0.14 mM) and high catalytic efficiency (V_max = 1.89 × 10⁻⁶ M·s⁻¹), enabling rapid oxidation of TMB to generate a colorimetric signal. Coupled with the DES-Aga hydrogel, the platform achieves dual-mode detection: laboratory-grade UV–vis quantification (detection limits: 0.01 μM HQ, 0.05 μM GSH, 0.02 μM NO₂⁻) and smartphone-assisted on-site analysis. The hydrogel leverages redox/diazotization interactions to produce distinct color transitions (blue → colorless for HQ/GSH; blue → yellow for NO₂⁻), validated in real-world matrices (cosmetics, food, serum) with recoveries of 87–115% and RSD < 8.6%. Key innovations include the nanozyme’s magnetic recyclability (> 80% activity after 7 cycles), the hydrogel’s stability (> 90% retention after 7 days), and a ratiometric strategy for NO₂⁻ detection. This work bridges the gap between laboratory precision and field-deployable diagnostics, offering a versatile tool for monitoring carcinogens in consumer products, food contaminants, and oxidative stress biomarkers, with direct implications for public health and safety.

Graphical abstract

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双模式纳米酶-水凝胶平台用于生物标志物和有害物质的现场多靶点检测

报道了一种将磁核壳型CuFe2O4/Cu/MnO2纳米酶与刺激响应型琼脂糖-深度共晶溶剂水凝胶（DES-Aga）集成在一起的新型双模传感系统。该纳米酶具有较低的米切里斯常数（Km = 0.14 mM）和较高的催化效率（Vmax = 1.89 × 10−6 M·s−1），能够快速氧化TMB并产生比色信号。结合DES-Aga水凝胶，该平台实现了双模式检测：实验室级紫外可见定量（检出限：0.01 μM HQ, 0.05 μM GSH, 0.02 μM NO2−）和智能手机辅助现场分析。水凝胶利用氧化还原/重氮化相互作用产生不同的颜色转换（HQ/GSH为蓝色→无色；NO2 -为蓝色→黄色），在现实世界的基质（化妆品，食品，血清）中得到验证，回收率为87-115%，RSD < 8.6%。关键的创新包括纳米酶的磁性可回收性（7个循环后活性为80%），水凝胶的稳定性（7天后保留率为90%），以及用于NO2 -检测的比率策略。这项工作弥补了实验室精度和现场可部署诊断之间的差距，为监测消费品、食品污染物和氧化应激生物标志物中的致癌物提供了一种多功能工具，对公共卫生和安全具有直接影响。图形抽象

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.