Transglycosylases in peptidoglycan biosynthesis: advances in structure, function, and antimicrobial development

IF 9.7 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Science China Chemistry Pub Date : 2025-07-01 DOI:10.1007/s11426-024-2601-y

Xiao-Lin Zhang, Han Ding, Aoxin Guo, Xue-Wei Liu

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引用次数: 0

Abstract

Peptidoglycan (PG), the essential exoskeleton in most bacteria, is synthesized through the action of bacterial transglycosylases (TGases), positioning these enzymes as elegant and desirable targets for antibiotic discovery. This review covers the major TGases involved in PG biogenesis, including TGases from the glycosyltransferase family 51 (GT51) and the newly discovered shape, elongation, division, sporulation (SEDS) family. We discuss the distinct roles of these two TGases during PG synthesis and emphasize the structural and catalytic differences, highlighting their coordination in PG assembly. Moreover, we summarize recent advances in TGase-involved antimicrobial strategies, including substrate-mimicking TGase inhibitors, PG terminators, and TGase-related immunological therapy targeting TGase from the GT51 family, and the first non-substrate-like TGase inhibitor against the SEDS protein. These valuable insights pave the way for the further development of novel TGase-related antimicrobial agents.

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肽聚糖生物合成中的转糖基酶：结构、功能和抗菌研究进展

肽聚糖（PG）是大多数细菌必不可少的外骨骼，通过细菌转糖基酶（tgase）的作用合成，使这些酶成为抗生素发现的优雅和理想靶点。本文综述了参与PG生物发生的主要tgase，包括糖基转移酶家族51 （GT51）和新发现的形状、延伸、分裂、产孢（SEDS）家族tgase。我们讨论了这两种tgase在PG合成过程中的不同作用，并强调了结构和催化的差异，强调了它们在PG组装中的协同作用。此外，我们总结了TGase相关抗菌策略的最新进展，包括底物模拟TGase抑制剂、PG终止物、针对GT51家族TGase的TGase相关免疫治疗，以及首个针对SEDS蛋白的非底物样TGase抑制剂。这些有价值的见解为进一步开发新型tgase相关抗菌剂铺平了道路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Science China Chemistry CHEMISTRY, MULTIDISCIPLINARY-

CiteScore

14.40

自引率

7.30%

发文量

3787

审稿时长

2.2 months

期刊介绍： Science China Chemistry, co-sponsored by the Chinese Academy of Sciences and the National Natural Science Foundation of China and published by Science China Press, publishes high-quality original research in both basic and applied chemistry. Indexed by Science Citation Index, it is a premier academic journal in the field. Categories of articles include: Highlights. Brief summaries and scholarly comments on recent research achievements in any field of chemistry. Perspectives. Concise reports on thelatest chemistry trends of interest to scientists worldwide, including discussions of research breakthroughs and interpretations of important science and funding policies. Reviews. In-depth summaries of representative results and achievements of the past 5–10 years in selected topics based on or closely related to the research expertise of the authors, providing a thorough assessment of the significance, current status, and future research directions of the field.