Effects of butyric acid on the inhibition of Vibrio parahaemolyticus and its impacts on growth performance, intestinal microbiota, and water microbiota in Litopenaeus vannamei

IF 3.7 2区 农林科学 Q1 FISHERIES
Minghan Yin , Zhangyi Zheng , Ruiyan Xing , Lefei Jiao , Moussa Gouife , Tinghong Ming , Fei Kong , Shuang Qiu , Jiajie Xu
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引用次数: 0

Abstract

Pathogenic Vibrio species have long posed a major challenge in shrimp aquaculture, while traditional antibiotic treatments are associated with numerous limitations, creating an urgent demand for novel preventive and therapeutic approaches. The present study employed in vivo and in vitro experiments to comprehensively evaluate the feasibility of butyric acid supplementation in Litopenaeus vannamei culture. In vitro antimicrobial assays demonstrated that under acidic conditions (pH 6), the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of butyric acid against V. parahaemolyticus were 20 mM and 40 mM, respectively. In contrast, under neutral conditions (pH 7), the MIC and MBC increased to 320 mM and 640 mM. At both MIC and MBC concentrations, butyric acid completely suppressed bacterial growth and significantly disrupted biofilm formation and cellular integrity. In vivo shrimp feeding trials revealed that dietary supplementation with 0.5 and 2.0 g/kg butyric acid significantly enhanced percent weight gain (PWG) and specific growth rate (SGR) (P < 0.05). 0.5 g/kg butyric acid supplementation could reduce the feed conversion ratio (P < 0.05). The addition of sodium butyrate (in its sodium salt form) significantly reduced the abundance of intestinal Vibrio, decreasing from 17.45 in the CK group to 8.63 %(SB0.5), 5.54 %(SB2.0), and 0.62 %(SB5.0) in the respective treatment groups, while promoting the proliferation of beneficial bacteria, particularly Verrucomicrobiota (P < 0.05). Additionally, butyric acid supplementation significantly decreased the relative abundance of Candidatus Aquiluna and Tenacibaculum in aquaculture water (P < 0.05). KEGG pathway of water microbiota showed that dietary supplementation with 0.5 and 2.0 g/kg sodium butyrate markedly exhibited significant enrichment in prodigiosin biosynthesis, while 5.0 g/kg butyric acid supplementation showed a marked reduction in the Thiamine metabolism pathway, which is critical for energy metabolism (P < 0.05). This study not only offered a novel feed additive option for healthy shrimp aquaculture but also established a theoretical foundation for its application.
丁酸对凡纳滨对虾副溶血性弧菌的抑制作用及其对生长性能、肠道菌群和水中菌群的影响
致病性弧菌长期以来一直是对虾养殖的主要挑战,而传统的抗生素治疗存在许多局限性,迫切需要新的预防和治疗方法。本研究通过体内和体外实验,综合评价凡纳滨对虾培养中添加丁酸的可行性。体外抗菌实验表明,在酸性条件下(pH 6),丁酸对副溶血性弧菌的最小抑菌浓度(MIC)和最小杀菌浓度(MBC)分别为20 mM和40 mM。而在中性条件下(pH 7), MIC和MBC分别增加到320 mM和640 mM。在MIC和MBC浓度下,丁酸完全抑制细菌生长,并显著破坏生物膜的形成和细胞完整性。体内饲养试验表明,饲粮中添加0.5和2.0 g/kg丁酸可显著提高对虾增重率(PWG)和特定生长率(SGR) (P <; 0.05)。添加0.5 g/kg丁酸可降低饲料系数(P <; 0.05)。丁酸钠(以钠盐形式)的添加显著降低了肠道弧菌的丰度,从CK组的17.45%降至8.63 %(SB0.5)、5.54 %(SB2.0)和0.62 %(SB5.0),同时促进了有益菌,特别是Verrucomicrobiota的增殖(P <; 0.05)。此外,添加丁酸显著降低了养殖水体中Aquiluna候选菌和Tenacibaculum的相对丰度(P <; 0.05)。水中微生物群的KEGG途径显示,饲粮中添加0.5和2.0 g/kg丁酸钠显著增强了虾仔虾灵菌素的生物合成,而饲粮中添加5.0 g/kg丁酸显著降低了虾仔虾能量代谢的关键硫胺素代谢途径(P <; 0.05)。本研究不仅为健康养殖对虾提供了一种新的饲料添加剂选择,而且为其应用奠定了理论基础。
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来源期刊
Aquaculture Reports
Aquaculture Reports Agricultural and Biological Sciences-Animal Science and Zoology
CiteScore
5.90
自引率
8.10%
发文量
469
审稿时长
77 days
期刊介绍: Aquaculture Reports will publish original research papers and reviews documenting outstanding science with a regional context and focus, answering the need for high quality information on novel species, systems and regions in emerging areas of aquaculture research and development, such as integrated multi-trophic aquaculture, urban aquaculture, ornamental, unfed aquaculture, offshore aquaculture and others. Papers having industry research as priority and encompassing product development research or current industry practice are encouraged.
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