Green synthesis and bioactivity of peach hibiscus silver nanoparticles

Abstract

Petals of peach hibiscus (Hibiscus rosa-sinensis) were used in the Synthesis of silver nanoparticles. The biosynthesized peach hibiscus silver nanoparticles (PH-AgNPs) were defined by X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, Scanning Electron Microscopy (SEM) and Energy dispersive X-ray analysis (EDX). PH-AgNPs was validated by the color shift from pink to dark brown. UV–visible spectrophotometry (UV–vis) results showed a strong absorbance peak at 420 nm. XRD spectra confirmed the crystalline nature of the nanoparticles. FT-IR analysis confirmed the functional moieties responsible for the capping and stabilizing of AgNPs nanoparticles. SEM verified the nanoparticles spherical form, with an average size of 46.5 nm. The nature of the elements was found to be 60 % silver using EDX. Total phenolic content and flavonoid content in peaches hibiscus extract (PHE) was determined. Maximum radical scavenging efficacy of 99.82 % was observed in PH-AgNPs. The PH-AgNPs were found to possess potent antibacterial activity against Klebsiella pneumonia, Escherichia coli, Enterococcus faecalis and Staphylococcus aureus. PH-AgNPs showed potent antidiabetic activity and anti-inflammatory activities. Cytotoxicity was evaluated by larvicidal activity of Artemia nauplii. MTT assay of the PH-AgNPs showed potent inhibitory effect on the A549 lung cancer cell lines with the IC₅₀ value 50.070 ± 1.685 µg/mL.