Comparative analysis of endogenous phytohormone profilings in different life stages of Haematococcus pluvialis by targeted metabolomics

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts Pub Date : 2025-08-31 DOI:10.1016/j.algal.2025.104283

Yang Sun , Yun Li , Jing Zhang , Jiaji Zhang , Wenjie Yan , Xu Gao

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引用次数: 0

Abstract

Phytohormones play a crucial role in the growth and production of commercial microalgae, and are closely associated with their life stages. In the study, targeted metabolomics analysis was performed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) to investigate the phytohormone profiles of four life stages (zoospores, ZP; palmella, PL; palmella cell in transition to aplanospore, PAL; aplanospore, AL) of Haematococcus pluvialis. A total of 18 phytohormones were identified and quantified, including 4 auxins, 8 cytokinins (CKs), 2 jasmonates, 2 growth inhibitors, 1 abscisic acid (ABA), and 1 salicylic acid (SA). Indole-3-acetic acid (IAA) was the major auxin, with the highest content observed at the PL stage when biomass accumulation reaches its highest peak. Cis-zeatin (cZ) and cis-zeatin riboside (cZR) were the two predominant CKs, which increased significantly at the AL stage. Similarly, abscisic acid (ABA) content was the highest at the AL stage, which was associated with the accumulation of astaxanthin. The results provide important basic information for the development of plant growth regulators in the production of H. pluvialis.

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利用靶向代谢组学方法对雨红球菌不同生命阶段内源植物激素谱进行比较分析

植物激素在商业微藻的生长和生产中起着至关重要的作用，并且与微藻的生命阶段密切相关。本研究采用液相色谱-串联质谱（LC-MS/MS）对雨红球菌（Haematococcus pluvialis）四个生命阶段（游动孢子，ZP；掌部，PL；掌部细胞向平流层孢子过渡，PAL；平流层孢子，AL）的植物激素谱进行了靶向代谢组学分析。共鉴定并定量鉴定了18种植物激素，包括4种生长素、8种细胞分裂素（ck）、2种茉莉酸盐、2种生长抑制剂、1种脱落酸（ABA）和1种水杨酸（SA）。吲哚-3-乙酸（IAA）是主要的生长素，在生物量积累达到高峰的PL期含量最高。顺式玉米蛋白（cZ）和顺式玉米蛋白核苷（cZR）是两种主要的ck，在AL期显著增加。ABA含量在AL期最高，这与虾青素的积累有关。研究结果为雨杉生产中植物生长调节剂的开发提供了重要的基础信息。

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来源期刊

Algal Research-Biomass Biofuels and Bioproducts BIOTECHNOLOGY & APPLIED MICROBIOLOGY-

CiteScore

9.40

自引率

7.80%

发文量

332

期刊介绍： Algal Research is an international phycology journal covering all areas of emerging technologies in algae biology, biomass production, cultivation, harvesting, extraction, bioproducts, biorefinery, engineering, and econometrics. Algae is defined to include cyanobacteria, microalgae, and protists and symbionts of interest in biotechnology. The journal publishes original research and reviews for the following scope: algal biology, including but not exclusive to: phylogeny, biodiversity, molecular traits, metabolic regulation, and genetic engineering, algal cultivation, e.g. phototrophic systems, heterotrophic systems, and mixotrophic systems, algal harvesting and extraction systems, biotechnology to convert algal biomass and components into biofuels and bioproducts, e.g., nutraceuticals, pharmaceuticals, animal feed, plastics, etc. algal products and their economic assessment