Prospective study on combined use of Sabouraud dextrose agar and blood agar for improved recovery of etiological agents in mycotic and Pythium keratitis

IF 1.9 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of microbiological methods Pub Date : 2025-09-05 DOI:10.1016/j.mimet.2025.107247

Harsimran Kaur , Mahuya Roy , Imola Jamir , Sukriti Yadav , Amit Gupta , Anup Ghosh , Archana Angrup , Shivaprakash M. Rudramurthy

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引用次数: 0

Abstract

We evaluated the effectiveness of using blood agar (BA) and Sabouraud dextrose agar (SDA) together to isolate fungi and Pythium insidiosum for the diagnosis of fungal and Pythium keratitis respectively. The overall recovery rate was higher in SDA than BA (93.75 % vs 88.28 %; p = 0.595). BA demonstrated lower isolation of dematiaceous fungi (unadjusted p = 0.039: FDR adjusted p = 0.062). 11.71 % and 6.25 % of fungal isolates were not recovered on BA and SDA, respectively. While BA showed faster growth of fungi than SDA (unadjusted p = 0.002; FDR adjusted p = 0.005), both BA and SDA showed rapid recovery of Aspergillus spp. as compared to Fusarium spp. (unadjusted p < 0.001; FDR adjusted p = 0.004). The combined use of both media may enhance detection of certain fungi missed by either medium alone, hence guiding the appropriate patient management.

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沙乌地葡萄糖琼脂与血琼脂联合应用对真菌性角膜炎和皮癣性角膜炎病原菌恢复的前瞻性研究

我们分别用血琼脂（BA）和Sabouraud葡萄糖琼脂（SDA）分离真菌和内生皮霉（Pythium insidiosum）对真菌性角膜炎和角膜炎皮霉（Pythium keratitis）进行了诊断。SDA的总回收率高于BA （93.75% vs 88.28%; p = 0.595）。BA显示较低的真菌分离率（未校正p = 0.039； FDR校正p = 0.062）。在BA和SDA上分别有11.71%和6.25%的真菌分离株未被恢复。BA的真菌生长速度快于SDA（未校正p = 0.002； FDR校正p = 0.005）， BA和SDA的曲霉菌恢复速度都快于镰刀菌（未校正p <； 0.001； FDR校正p = 0.004）。两种培养基的联合使用可以提高对单独使用一种培养基所遗漏的某些真菌的检测，从而指导适当的患者管理。

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来源期刊

Journal of microbiological methods 生物-生化研究方法

CiteScore

4.30

自引率

4.50%

发文量

151

审稿时长

29 days

期刊介绍： The Journal of Microbiological Methods publishes scholarly and original articles, notes and review articles. These articles must include novel and/or state-of-the-art methods, or significant improvements to existing methods. Novel and innovative applications of current methods that are validated and useful will also be published. JMM strives for scholarship, innovation and excellence. This demands scientific rigour, the best available methods and technologies, correctly replicated experiments/tests, the inclusion of proper controls, calibrations, and the correct statistical analysis. The presentation of the data must support the interpretation of the method/approach. All aspects of microbiology are covered, except virology. These include agricultural microbiology, applied and environmental microbiology, bioassays, bioinformatics, biotechnology, biochemical microbiology, clinical microbiology, diagnostics, food monitoring and quality control microbiology, microbial genetics and genomics, geomicrobiology, microbiome methods regardless of habitat, high through-put sequencing methods and analysis, microbial pathogenesis and host responses, metabolomics, metagenomics, metaproteomics, microbial ecology and diversity, microbial physiology, microbial ultra-structure, microscopic and imaging methods, molecular microbiology, mycology, novel mathematical microbiology and modelling, parasitology, plant-microbe interactions, protein markers/profiles, proteomics, pyrosequencing, public health microbiology, radioisotopes applied to microbiology, robotics applied to microbiological methods,rumen microbiology, microbiological methods for space missions and extreme environments, sampling methods and samplers, soil and sediment microbiology, transcriptomics, veterinary microbiology, sero-diagnostics and typing/identification.