Vasiliki E. Mourikes, Colin Lee, Jadesola I. Oladosu, Ashley Deviney, Winter Stubblefield, Mary J. Laws, Megan Mahoney, Jodi A. Flaws
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引用次数: 0
Abstract
Imidacloprid (IMI) is an insect-selective synthetic nicotine derivative used in commercial agricultural systems, home gardening, and veterinary pharmaceuticals. People are exposed to IMI through consumption of contaminated food and water and through contact with companion animals. However, the effects of IMI on the female reproductive system are not well understood. Thus, we tested the hypothesis that IMI reaches the ovaries and adversely affects the female reproductive system. Adult female mice were orally exposed to vehicle control (dimethyl sulfoxide) or IMI for 30 days. Estrous cyclicity was monitored for the last 14 days of the dosing period and mice were euthanized in diestrus. Sera were collected for gonadotropin and sex steroid hormone quantification. Ovaries were collected for IMI and IMI metabolite quantification, and to assess follicle numbers and gene expression. IMI and metabolites were significantly higher in exposed mice compared to controls. Further, IMI decreased the number of healthy ovarian follicles and increased the ovarian expression of the enzymes Cyp2e1 and Cyp19a1 compared to control. IMI also increased circulating luteinizing hormone levels (LH), but did not affect circulating follicle-stimulating hormone levels or sex steroid hormone levels compared to control. IMI did not affect body weight, ovarian weight, or estrous cyclicity compared to control. Collectively, these data indicate that IMI reaches the ovaries and affects some female reproductive outcomes such as ovarian follicle numbers, LH levels, and ovarian expression of enzymes.
期刊介绍:
Drawing from a large number of disciplines, Reproductive Toxicology publishes timely, original research on the influence of chemical and physical agents on reproduction. Written by and for obstetricians, pediatricians, embryologists, teratologists, geneticists, toxicologists, andrologists, and others interested in detecting potential reproductive hazards, the journal is a forum for communication among researchers and practitioners. Articles focus on the application of in vitro, animal and clinical research to the practice of clinical medicine.
All aspects of reproduction are within the scope of Reproductive Toxicology, including the formation and maturation of male and female gametes, sexual function, the events surrounding the fusion of gametes and the development of the fertilized ovum, nourishment and transport of the conceptus within the genital tract, implantation, embryogenesis, intrauterine growth, placentation and placental function, parturition, lactation and neonatal survival. Adverse reproductive effects in males will be considered as significant as adverse effects occurring in females. To provide a balanced presentation of approaches, equal emphasis will be given to clinical and animal or in vitro work. Typical end points that will be studied by contributors include infertility, sexual dysfunction, spontaneous abortion, malformations, abnormal histogenesis, stillbirth, intrauterine growth retardation, prematurity, behavioral abnormalities, and perinatal mortality.