Identification, and Characterization of Escherichia coli O157:H7 from Animal-Derived Foods in Hawassa and Yirgalem, Sidama Region, Ethiopia

IF 2.8 4区农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of food protection Pub Date : 2025-09-04 DOI:10.1016/j.jfp.2025.100614

Gemechu Chala , Workagegnew Israel , Alemayehu Gebeyehu , Wubishet Mulugeta , Getachew Ngusie , Senait Belay

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引用次数: 0

Abstract

Escherichia coli O157:H7 is a significant foodborne pathogen with global public health implications. This study, conducted from December 2022 to July 2023 in Hawassa and Yirgalem, Sidama Region, Ethiopia, assessed the prevalence, molecular identification, and antimicrobial resistance of E. coli O157:H7 in animal-derived foods. A total of 298 samples including beef, raw milk, and fish were analyzed using culture methods, biochemical identification, and PCR. The overall prevalence of E. coli O157:H7 was 3.7%, with isolation rates of 6.25% in fish and 5.6% in beef; no isolates were found in milk samples (p = 0.036). Notably, meat from butcher shops exhibited a higher contamination rate (7.6%) compared to abattoir samples (3.4%), though this difference was not statistically significant (p = 0.445). Among fish samples, Nile Tilapia had a higher isolation rate (8.1%) than African Catfish (3.7%), with fish skin showing greater contamination (8.6%) than muscle tissue (3.4%). All E. coli O157:H7 isolates were susceptible to most tested antibiotics; however, resistance was observed against amoxicillin (81.8%), streptomycin (45.5%), and clindamycin (100%). Additionally, 45.5% of isolates exhibited multidrug resistance with an multidrug resistance (MDR) index of 0.27, though none produced extended-spectrum beta-lactamases (ESBLs). These findings underscore the necessity for stringent hygiene practices and effective monitoring of animal-derived foods to mitigate public health risks.

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埃塞俄比亚西达马地区Hawassa和Yirgalem动物源性食品中大肠杆菌O157:H7的鉴定和鉴定

大肠杆菌O157:H7是一种具有全球公共卫生影响的重要食源性病原体。该研究于2022年12月至2023年7月在埃塞俄比亚Sidama地区的Hawassa和Yirgalem进行，评估了动物源性食品中大肠杆菌O157:H7的流行情况、分子鉴定和耐药性。通过培养、生化鉴定、PCR等方法，对牛肉、鲜奶、鱼类等共298份样品进行了分析。大肠杆菌O157:H7的总流行率为3.7%，其中鱼类和牛肉的分离率分别为6.25%和5.6%；牛奶样品中未发现分离菌（p = 0.036）。值得注意的是，与屠宰场的样本（3.4%）相比，肉店的肉显示出更高的污染率（7.6%），尽管这种差异没有统计学意义（p = 0.445）。在鱼类样本中，尼罗罗非鱼的分离率（8.1%）高于非洲鲶鱼（3.7%），鱼皮污染（8.6%）高于肌肉组织（3.4%）。所有O157:H7大肠杆菌分离株对大多数所测抗生素均敏感；但对阿莫西林（81.8%）、链霉素（45.5%）和克林霉素（100%）耐药。此外，45.5%的菌株表现出多药耐药（MDR）指数为0.27，但没有菌株产生广谱β -内酰胺酶（ESBL）。这些发现强调了严格的卫生习惯和对动物源性食品进行有效监测以减轻公共卫生风险的必要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of food protection 工程技术-生物工程与应用微生物

CiteScore

4.20

自引率

5.00%

发文量

296

审稿时长

2.5 months

期刊介绍： The Journal of Food Protection® (JFP) is an international, monthly scientific journal in the English language published by the International Association for Food Protection (IAFP). JFP publishes research and review articles on all aspects of food protection and safety. Major emphases of JFP are placed on studies dealing with: Tracking, detecting (including traditional, molecular, and real-time), inactivating, and controlling food-related hazards, including microorganisms (including antibiotic resistance), microbial (mycotoxins, seafood toxins) and non-microbial toxins (heavy metals, pesticides, veterinary drug residues, migrants from food packaging, and processing contaminants), allergens and pests (insects, rodents) in human food, pet food and animal feed throughout the food chain; Microbiological food quality and traditional/novel methods to assay microbiological food quality; Prevention of food-related hazards and food spoilage through food preservatives and thermal/non-thermal processes, including process validation; Food fermentations and food-related probiotics; Safe food handling practices during pre-harvest, harvest, post-harvest, distribution and consumption, including food safety education for retailers, foodservice, and consumers; Risk assessments for food-related hazards; Economic impact of food-related hazards, foodborne illness, food loss, food spoilage, and adulterated foods; Food fraud, food authentication, food defense, and foodborne disease outbreak investigations.