Tailoring the Mechanical and Functional Properties of Lupin Protein Films by pH Manipulation, Acetic Acid, and Ultrasonic Treatment

IF 5 3区工程技术 Q2 ENGINEERING, ENVIRONMENTAL

Journal of Polymers and the Environment Pub Date : 2025-07-17 DOI:10.1007/s10924-025-03638-w

Maximilian Maidl, Daniel Van Opdenbosch, Cordt Zollfrank

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引用次数: 0

Abstract

The ability of proteins to self-assemble into ordered structures is a key feature in nature to produce highly functional biomacromolecules. This spontaneous organization of polypeptides can be utilized in technology to develop environment-friendly, protein-based bioplastic films with enhanced properties from various proteins. This study focuses on tailoring the mechanical and functional properties of solvent-cast films by pH value manipulation (10, 7, 2), acetic acid treatment, and ultrasonication of heated (85 °C, 30 min) solutions of lupin protein isolate (LPI). A combination of acetic acid and ultrasonic treatment yielded LPI films that exhibited increased tensile strengths of 6.8 ± 0.4 MPa, Young’s moduli of 270 ± 18 MPa, and works of fracture of 4.4 ± 0.5 MPa, together with high optical transmissions and water stability compared to the other investigated films. Structural investigations indicated the formation of self-assembled protein nanostructures with an increased fraction of β-sheets. Small-angle X-ray scattering investigations were performed to gain insights into the nanostructural evolution of protein aggregates from LPI during the evaporation of water.

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通过pH值处理、醋酸和超声波处理来调整罗苹蛋白薄膜的机械和功能特性

蛋白质自组装成有序结构的能力是自然界中产生高功能生物大分子的关键特征。这种多肽的自发组织可以在技术上用于开发环境友好的、基于蛋白质的生物塑料薄膜，这些薄膜具有各种蛋白质的增强性能。本研究的重点是通过pH值控制（10,7,2），醋酸处理和加热（85°C， 30分钟）的罗苹分离蛋白（LPI）溶液的超声波处理来定制溶剂铸造膜的机械和功能特性。与其他研究的薄膜相比，醋酸和超声波联合处理得到的LPI薄膜的抗拉强度增加了6.8±0.4 MPa，杨氏模量增加了270±18 MPa，断裂功增加了4.4±0.5 MPa，同时具有较高的光透射率和水稳定性。结构研究表明，自组装蛋白质纳米结构的形成与β-片的比例增加。小角度x射线散射研究是为了深入了解LPI在水分蒸发过程中蛋白质聚集体的纳米结构演变。

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来源期刊

Journal of Polymers and the Environment 工程技术-高分子科学

CiteScore

9.50

自引率

7.50%

发文量

297

审稿时长

9 months

期刊介绍： The Journal of Polymers and the Environment fills the need for an international forum in this diverse and rapidly expanding field. The journal serves a crucial role for the publication of information from a wide range of disciplines and is a central outlet for the publication of high-quality peer-reviewed original papers, review articles and short communications. The journal is intentionally interdisciplinary in regard to contributions and covers the following subjects - polymers, environmentally degradable polymers, and degradation pathways: biological, photochemical, oxidative and hydrolytic; new environmental materials: derived by chemical and biosynthetic routes; environmental blends and composites; developments in processing and reactive processing of environmental polymers; characterization of environmental materials: mechanical, physical, thermal, rheological, morphological, and others; recyclable polymers and plastics recycling environmental testing: in-laboratory simulations, outdoor exposures, and standardization of methodologies; environmental fate: end products and intermediates of biodegradation; microbiology and enzymology of polymer biodegradation; solid-waste management and public legislation specific to environmental polymers; and other related topics.