Karyoevolutionary processes in Atlantic damselfishes of the genus Stegastes (Pomacentridae).

IF 1.3 4区 生物学 Q4 CELL BIOLOGY
Marsílio S P Rocha, Gideão W W F Costa, Marcelo B Cioffi, Luiz A C Bertollo, Vanessa C S Oliveira, Karlla D J Amorim, Wagner F Molina
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引用次数: 0

Abstract

Background: The damselfishes, an extremely diverse group of herbivorous fish, stands out as an important and ubiquitous ecological component of coral reefs. In the Western South Atlantic, the genus Stegastes is the most representative, whose evolutionary paths and taxonomic status of insular endemic species have been better evaluated. To clarify the karyotypic evolution involved in the diversification of this group, cytogenetic analyses were performed in four nominal species (S. variabilis and S. fuscus, distributed in Brazilian coastal regions; S. rocasensis and S. sanctipauli, from Rocas Atoll and São Paulo and São Pedro Archipelago) and one subspecies (S. fuscus trindadensis, from Trindade and Martim Vaz Archipelago).

Results: Classical cytogenetic protocols and fluorescence in situ hybridization (FISH) with 18S and 5S rDNA probes, were used for comparative analyses. All species had 2n=48 chromosomes, with high FN values ranging from 88 to 92. Stegastes rocasensis and S. sanctipauli shared identical cytogenetic patterns, while S. f. trindadensis revealed a syntenic arrangement of 18S and 5S rDNA sites not found in S. fuscus from the Brazilian coast.

Conclusion: The karyotypic evolution of Stegastes was predominantly driven by multiple pericentric inversions (and/or centromere shifts), resulting in changes in the internal organization of chromosomes. Stegastes rocasensis and S. sanctipauli have similar cytogenetical patterns, as well as S. fuscus and S. f. trindadensis indicating incipient evolutionary differentiation in insular species. Mapping other repetitive DNA sequences provided an exceptional opportunity to clarify chromosomal changes and their association with the evolutionary diversification of Stegastes species.

大西洋豆娘鱼(豆娘科)的核进化过程。
豆娘鱼是一种极其多样化的草食性鱼类,是珊瑚礁中重要的、无处不在的生态组成部分。在南大西洋西部,Stegastes属是最具代表性的,其进化路径和岛屿特有种的分类地位已得到较好的评价。为了阐明这一群体多样化的核型进化,对分布在巴西沿海地区的4个名义种(S. variabilis和S. fuscus)、来自罗卡斯环礁和圣保罗和 o Pedro群岛的S. rocasensis和S. sanctipauli)和一个亚种(来自Trindade和Martim Vaz群岛的S. fuscus trindadensis)进行了细胞遗传学分析。结果:采用经典细胞遗传学方法和18S和5S rDNA探针的荧光原位杂交(FISH)进行比较分析。所有物种有2n=48条染色体,高FN值在88 ~ 92之间。rocasensis与S. sanctipauli具有相同的细胞遗传模式,而S. f. trindadensis在巴西海岸的S. fuscus中没有发现18S和5S rDNA位点的同步排列。结论:Stegastes的核型进化主要是由多个中心倒位(和/或着丝粒移位)驱动的,从而导致染色体内部组织的变化。rocasensis和S. sanctipauli具有相似的细胞遗传模式,S. fuscus和S. trindadensis也具有相似的细胞遗传模式,表明岛屿物种的进化分化初期。绘制其他重复DNA序列提供了一个特殊的机会来澄清染色体变化及其与Stegastes物种进化多样化的关系。
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来源期刊
Cytogenetic and Genome Research
Cytogenetic and Genome Research 生物-细胞生物学
CiteScore
3.10
自引率
5.90%
发文量
25
审稿时长
1 months
期刊介绍: During the last decades, ''Cytogenetic and Genome Research'' has been the leading forum for original reports and reviews in human and animal cytogenetics, including molecular, clinical and comparative cytogenetics. In recent years, most of its papers have centered on genome research, including gene cloning and sequencing, gene mapping, gene regulation and expression, cancer genetics, comparative genetics, gene linkage and related areas. The journal also publishes key papers on chromosome aberrations in somatic, meiotic and malignant cells. Its scope has expanded to include studies on invertebrate and plant cytogenetics and genomics. Also featured are the vast majority of the reports of the International Workshops on Human Chromosome Mapping, the reports of international human and animal chromosome nomenclature committees, and proceedings of the American and European cytogenetic conferences and other events. In addition to regular issues, the journal has been publishing since 2002 a series of topical issues on a broad variety of themes from cytogenetic and genome research.
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