Precision in Spinal Cord Injury Research: A Novel Electromagnetic Impactor for a Consistent Porcine Model.

IF 5.4 2区医学 Q3 ENGINEERING, BIOMEDICAL

Annals of Biomedical Engineering Pub Date : 2025-09-05 DOI:10.1007/s10439-025-03836-6

Leonard Steger, Abdul Karim Ghaith, Carly Weber-Levine, Kayla Robinson, Christina Krueger, Constantin Smit, Siddharth Krishnan, Kelley M Kempski Leadingham, Daniel Davidar, Denis Routkevitch, Kelly Jiang, Victor Quiroz, Stuart Bauer, Ruixing Liang, Max Kerensky, Ian Suk, Betty Tyler, Joshua C Doloff, Nicholas Theodore, Amir Manbachi

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引用次数: 0

Abstract

Purpose: Replicating spinal cord injury (SCI) in large animals is necessary for evaluating translational therapeutics, yet there is currently no commercial, standardized device for inducing SCI. We present the fabrication and testing of a custom impactor device for producing repeatable contusion SCI in porcine models.

Methods: The device was built, and mechanical modeling was utilized for calibration. Benchtop verification measured impact force. Impact velocity and kinetic energy were calculated. The device was used to generate a contusion SCI model in 2 pigs and the results were compared to an uninjured pig. Ultrasound imaging and Hematoxylin-eosin (H&E) staining were used to confirm injury presence. The device was utilized in survival studies requiring a porcine SCI model and motor scores were collected at postoperative day 1 in 4 pigs.

Results: Characterization revealed distinct impact velocities for each dial turn. This device further demonstrated repeatability and the potential for modulating injury severity on the benchtop. Impactor forces were demonstrated across a range from 12.8 to 67.6 N, with error between 0.2 and 0.7 N. Kinetic energy ranged from 0.045 to 0.338 J, with error between 0.0009 to 0.003 J. Intraoperative ultrasound imaging and histology of the spinal cord confirmed two injuries of different severity. The device produced a variety of injury severities through parameter modifications in survival studies assessed by the porcine neurological motor (PNM) score.

Conclusion: This impactor device is a major advancement towards titratable contusions in large animal SCI models.

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脊髓损伤研究的精确性：一种用于一致性猪模型的新型电磁冲击器。

目的：在大动物身上复制脊髓损伤（SCI）是评估转化疗法的必要条件，但目前还没有商业化的、标准化的设备来诱导SCI。我们提出了一种定制的冲击装置的制造和测试，用于在猪模型中产生可重复的挫伤损伤。方法：建立装置，利用力学模型进行标定。台式验证测量的冲击力。计算了冲击速度和动能。用该装置制作2头猪的挫伤损伤模型，并与未受伤猪进行比较。超声成像和苏木精-伊红（H&E）染色证实损伤的存在。该装置用于需要猪脊髓损伤模型的生存研究，并在4头猪术后第1天收集运动评分。结果：表征揭示了不同的冲击速度为每一个转盘。该设备进一步证明了可重复性和在工作台上调节损伤严重程度的潜力。撞击力范围为12.8 - 67.6 N，误差在0.2 - 0.7 N之间。动能范围为0.045 - 0.338 J，误差在0.0009 - 0.003 J之间。术中超声成像和脊髓组织学证实了两种不同严重程度的损伤。该装置通过猪神经运动（PNM）评分评估的生存研究参数修改产生各种损伤严重程度。结论：该冲击器装置是大型动物脊髓损伤模型中可滴定挫伤的重要进展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Annals of Biomedical Engineering 工程技术-工程：生物医学

CiteScore

7.50

自引率

15.80%

发文量

212

审稿时长

3 months

期刊介绍： Annals of Biomedical Engineering is an official journal of the Biomedical Engineering Society, publishing original articles in the major fields of bioengineering and biomedical engineering. The Annals is an interdisciplinary and international journal with the aim to highlight integrated approaches to the solutions of biological and biomedical problems.