Role of Like Heterochromatin Protein1 (LHP1) in the root apical meristem and stem cell niche maintenance in Arabidopsis

IF 4.1 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science Pub Date : 2025-09-03 DOI:10.1016/j.plantsci.2025.112740

Gabriela Guzmán-Favila , M. Teresa Alejo-Vinogradova , Diego Ornelas-Ayala , José Olvera-Herrera , Rosario Vega-León , Bénédicte Desvoyes , Adriana Garay-Arroyo , Elena R. Alvarez-Buylla , Crisanto Gutierrez , Maria de la Paz Sanchez

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引用次数: 0

Abstract

Epigenetic regulation by Polycomb Group (PcG) is essential for controlling gene repression. In plants, PcG is involved in all developmental processes, from embryogenesis to floral development, including root development. LIKE HETEROCHROMATIN PROTEIN 1 (LHP1) has been described as a PcG component, capable of recognizing the H3K27me3 mark, that together with CLF, a PcG histone methyltransferase, represses gene expression. Here, we provide evidence that LHP1 plays different roles in the root apical meristem: a canonical role to repress gene expression in a similar way to CLF, and another where it participates in transcriptional activation, revealing a dual regulation of LHP1 that is tissue-specific. We also found novel roles for LHP1 in maintaining the root stem cell niche (RSCN), regulating columella stem cell (CSC) differentiation, auxin signaling and perception of light and gravitropic signals. Our findings integrate a novel epigenetic component into the regulatory network that maintains homeostasis of the RSCN.

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样异染色质蛋白1 （LHP1）在拟南芥根尖分生组织和干细胞生态位维持中的作用

Polycomb Group （PcG）的表观遗传调控是调控基因抑制的关键。在植物中，PcG参与了从胚胎发育到花发育，包括根发育的所有发育过程。LIKE异染色质蛋白1 （LHP1）已被描述为一种PcG成分，能够识别H3K27me3标记，与PcG组蛋白甲基转移酶CLF一起抑制基因表达。在这里，我们提供的证据表明LHP1在根尖分生组织中发挥着不同的作用：一个典型的作用是以类似于CLF的方式抑制基因表达，另一个则是参与转录激活，揭示了LHP1的组织特异性双重调控。我们还发现了LHP1在维持根干细胞生态位（RSCN）、调节小柱干细胞（CSC）分化、生长素信号传导以及对光和向地性信号的感知方面的新作用。我们的研究结果将一种新的表观遗传成分整合到维持RSCN稳态的调控网络中。

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来源期刊

Plant Science 生物-生化与分子生物学

CiteScore

9.10

自引率

1.90%

发文量

322

审稿时长

33 days

期刊介绍： Plant Science will publish in the minimum of time, research manuscripts as well as commissioned reviews and commentaries recommended by its referees in all areas of experimental plant biology with emphasis in the broad areas of genomics, proteomics, biochemistry (including enzymology), physiology, cell biology, development, genetics, functional plant breeding, systems biology and the interaction of plants with the environment. Manuscripts for full consideration should be written concisely and essentially as a final report. The main criterion for publication is that the manuscript must contain original and significant insights that lead to a better understanding of fundamental plant biology. Papers centering on plant cell culture should be of interest to a wide audience and methods employed result in a substantial improvement over existing established techniques and approaches. Methods papers are welcome only when the technique(s) described is novel or provides a major advancement of established protocols.