Metabolomic and transcriptomic analysis reveals high light to promote tuber enlargement through starch accumulation in Pinellia ternata

IF 4.5 Q1 PLANT SCIENCES
Duan Wu, Qin Chang, Minting Lu, Qi shen
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引用次数: 0

Abstract

Pinellia ternata (Thunb.) Breit, a vital traditional Chinese medicinal plant, responds distinctively to high light conditions. To reveal that light signal regulate material transport and accumulation in P. ternata, integrated analyses of metabolomic, hormone levels, full - length transcriptome sequencing, and RNA-seq were carried out. High light inhibits growth and chlorophyll accumulation of P. ternata, but activates the photoprotective system and significantly promoting tuber enlargement and increasing starch accumulation by 24.92 % in tubers. In tubers of P. ternata, 210 DAMs and 1459 DEGs were enriched in key pathways like photosynthesis, hormone signaling transduction and starch and sucrose metabolism. High light promotes the expression of genes that are involved in the synthesis of stress - responsive hormones such as JA, ABA, IAA and SA, as well as the expression of stress response factors (mTERF, GNAT) in the leaves of P. ternata, but reduces the accumulation of these hormone in tubers. Simultaneously, high light inhibits the expression of light signal-responsive genes (e.g., PIF4, CCA1, and PHYA) and upregulates genes involved in phototropism (PHOT2) and chlorophyll biosynthesis (e.g., GluTR, GSAM, UROD, COPRO genⅢ-Ox). Additionally, by activating the expression of genes encoding sugar transporters (pGlcTs, PMT, TMTs, SWEETs) and genes related to starch and sucrose synthases (SS, SPS, SBE, GBSSI, AGPase), high light facilitates the conversion of monosaccharides, including fructose and glucose, into starch for accumulation, thereby promoting the swelling of tubers. The proposed mechanism indicates high-light activation of photoprotection and energy conversion promotes sugar and photosynthetic product handling, facilitating tuber growth. This research offers novel insights into light - regulation in P. ternata, guiding its high - yield cultivation and enhancing understanding of its adaptation to high-light environments.
代谢组学和转录组学分析表明,强光通过淀粉积累促进半夏块茎膨大
半夏(Pinellia ternata)布雷特是一种重要的传统中药植物,对强光条件有独特的反应。为了揭示光信号对P. ternata物质转运和积累的调控作用,我们对其代谢组学、激素水平、全长转录组测序和RNA-seq进行了综合分析。强光抑制了紫菜的生长和叶绿素积累,但激活了光保护系统,显著促进了块茎膨大,增加了24.92 %的淀粉积累。在块茎中,210个dam和1459个deg在光合作用、激素信号转导、淀粉和蔗糖代谢等关键途径中富集。强光促进了叶片中JA、ABA、IAA、SA等胁迫应答激素合成相关基因的表达以及胁迫应答因子(mTERF、GNAT)的表达,但减少了这些激素在块茎中的积累。同时,强光抑制光信号响应基因(如PIF4、CCA1和PHYA)的表达,上调参与向光性(PHOT2)和叶绿素生物合成的基因(如GluTR、GSAM、UROD、COPRO genⅢ-Ox)。此外,通过激活编码糖转运蛋白的基因(pGlcTs, PMT, TMTs, SWEETs)和淀粉和蔗糖合成酶相关基因(SS, SPS, SBE, GBSSI, AGPase)的表达,强光促进了单糖(包括果糖和葡萄糖)转化为淀粉积累,从而促进块茎肿胀。该机制表明,高光激活的光保护和能量转换促进了糖和光合产物的处理,促进了块茎的生长。本研究为深入了解柽柳的光调控机制提供了新的思路,为柽柳高产栽培提供了指导,为柽柳对强光环境的适应提供了新的认识。
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来源期刊
Current Plant Biology
Current Plant Biology Agricultural and Biological Sciences-Plant Science
CiteScore
10.90
自引率
1.90%
发文量
32
审稿时长
50 days
期刊介绍: Current Plant Biology aims to acknowledge and encourage interdisciplinary research in fundamental plant sciences with scope to address crop improvement, biodiversity, nutrition and human health. It publishes review articles, original research papers, method papers and short articles in plant research fields, such as systems biology, cell biology, genetics, epigenetics, mathematical modeling, signal transduction, plant-microbe interactions, synthetic biology, developmental biology, biochemistry, molecular biology, physiology, biotechnologies, bioinformatics and plant genomic resources.
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