{"title":"Metabolomic and transcriptomic analysis reveals high light to promote tuber enlargement through starch accumulation in Pinellia ternata","authors":"Duan Wu, Qin Chang, Minting Lu, Qi shen","doi":"10.1016/j.cpb.2025.100529","DOIUrl":null,"url":null,"abstract":"<div><div><em>Pinellia ternata</em> (Thunb.) Breit, a vital traditional Chinese medicinal plant, responds distinctively to high light conditions. To reveal that light signal regulate material transport and accumulation in <em>P. ternata</em>, integrated analyses of metabolomic, hormone levels, full - length transcriptome sequencing, and RNA-seq were carried out. High light inhibits growth and chlorophyll accumulation of <em>P. ternata</em>, but activates the photoprotective system and significantly promoting tuber enlargement and increasing starch accumulation by 24.92 % in tubers. In tubers of <em>P. ternata</em>, 210 DAMs and 1459 DEGs were enriched in key pathways like photosynthesis, hormone signaling transduction and starch and sucrose metabolism. High light promotes the expression of genes that are involved in the synthesis of stress - responsive hormones such as JA, ABA, IAA and SA, as well as the expression of stress response factors (<em>mTERF</em>, <em>GNAT</em>) in the leaves of <em>P. ternata</em>, but reduces the accumulation of these hormone in tubers. Simultaneously, high light inhibits the expression of light signal-responsive genes (e.g., <em>PIF4</em>, <em>CCA1</em>, and <em>PHYA</em>) and upregulates genes involved in phototropism (<em>PHOT2</em>) and chlorophyll biosynthesis (e.g., <em>GluTR</em>, <em>GSAM</em>, <em>UROD</em>, <em>COPRO genⅢ-Ox</em>). Additionally, by activating the expression of genes encoding sugar transporters (<em>pGlcTs</em>, <em>PMT</em>, <em>TMTs</em>, <em>SWEETs</em>) and genes related to starch and sucrose synthases (<em>SS</em>, <em>SPS</em>, <em>SBE</em>, <em>GBSSI</em>, <em>AGPase</em>), high light facilitates the conversion of monosaccharides, including fructose and glucose, into starch for accumulation, thereby promoting the swelling of tubers. The proposed mechanism indicates high-light activation of photoprotection and energy conversion promotes sugar and photosynthetic product handling, facilitating tuber growth. This research offers novel insights into light - regulation in <em>P. ternata</em>, guiding its high - yield cultivation and enhancing understanding of its adaptation to high-light environments.</div></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"44 ","pages":"Article 100529"},"PeriodicalIF":4.5000,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Plant Biology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214662825000970","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Pinellia ternata (Thunb.) Breit, a vital traditional Chinese medicinal plant, responds distinctively to high light conditions. To reveal that light signal regulate material transport and accumulation in P. ternata, integrated analyses of metabolomic, hormone levels, full - length transcriptome sequencing, and RNA-seq were carried out. High light inhibits growth and chlorophyll accumulation of P. ternata, but activates the photoprotective system and significantly promoting tuber enlargement and increasing starch accumulation by 24.92 % in tubers. In tubers of P. ternata, 210 DAMs and 1459 DEGs were enriched in key pathways like photosynthesis, hormone signaling transduction and starch and sucrose metabolism. High light promotes the expression of genes that are involved in the synthesis of stress - responsive hormones such as JA, ABA, IAA and SA, as well as the expression of stress response factors (mTERF, GNAT) in the leaves of P. ternata, but reduces the accumulation of these hormone in tubers. Simultaneously, high light inhibits the expression of light signal-responsive genes (e.g., PIF4, CCA1, and PHYA) and upregulates genes involved in phototropism (PHOT2) and chlorophyll biosynthesis (e.g., GluTR, GSAM, UROD, COPRO genⅢ-Ox). Additionally, by activating the expression of genes encoding sugar transporters (pGlcTs, PMT, TMTs, SWEETs) and genes related to starch and sucrose synthases (SS, SPS, SBE, GBSSI, AGPase), high light facilitates the conversion of monosaccharides, including fructose and glucose, into starch for accumulation, thereby promoting the swelling of tubers. The proposed mechanism indicates high-light activation of photoprotection and energy conversion promotes sugar and photosynthetic product handling, facilitating tuber growth. This research offers novel insights into light - regulation in P. ternata, guiding its high - yield cultivation and enhancing understanding of its adaptation to high-light environments.
期刊介绍:
Current Plant Biology aims to acknowledge and encourage interdisciplinary research in fundamental plant sciences with scope to address crop improvement, biodiversity, nutrition and human health. It publishes review articles, original research papers, method papers and short articles in plant research fields, such as systems biology, cell biology, genetics, epigenetics, mathematical modeling, signal transduction, plant-microbe interactions, synthetic biology, developmental biology, biochemistry, molecular biology, physiology, biotechnologies, bioinformatics and plant genomic resources.