Elevated carbon dioxide enhances polyunsaturated fatty acids in Isochrysis galbana and improves rotifer growth through algal feeding

IF 3.7 1区 地球科学 Q1 LIMNOLOGY
Yitong Wang, Xi Zhu, Yuna Wang, Xingxing Cai, Hao Long, Aiyou Huang, Yanhua Zeng, Wei Ren, Zhenyu Xie, Xiaoni Cai
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引用次数: 0

Abstract

Elevated atmospheric CO2 levels directly affect marine primary producers by altering their photosynthetic efficiency and biochemical composition, thereby modifying nutrient transfer throughout marine food webs. This study examines the physiological, biochemical, and transcriptomic responses of the microalga Isochrysis galbana when cultured under elevated CO2 level (1000 μatm CO2) and current ambient CO2 level (420 μatm CO2) conditions, as well as the subsequent effects of feeding these algae to rotifer (Brachionus plicatilis). Under high CO2 conditions, I. galbana exhibited enhanced photosynthetic efficiency, carbon fixation, and energy metabolism, driven by the upregulation of genes involved in photosynthesis, the Calvin‐Benson cycle, and glycolysis. These molecular adaptations increased the growth rate by 24% and increased the content of polyunsaturated fatty acids (PUFAs) by 8%, including docosahexaenoic acid, which improves the nutritional quality of the alga. Feeding experiments demonstrated that rotifers fed with I. galbana grown under high‐CO2 conditions exhibited improved growth and enriched PUFA profiles, highlighting the potential for high‐CO2‐induced changes in primary producers to enhance trophic transfer efficiency. This study provides new insights into the molecular mechanisms underlying I. galbana's response to elevated CO2 and its ecological implications. The findings emphasize the capacity of marine microalgae to adapt to environmental changes and highlight the cascading effects of elevated CO2 on marine food web nutrition and dynamics.
升高的二氧化碳增加了等chrysis galbana中的多不饱和脂肪酸,并通过藻类喂养改善轮虫的生长
大气二氧化碳浓度升高通过改变海洋初级生产者的光合效率和生化组成,从而改变整个海洋食物网的营养转移,从而直接影响海洋初级生产者。本研究考察了高CO2浓度(1000 μatm CO2)和低CO2浓度(420 μatm CO2)条件下galbana等chrysis微藻的生理、生化和转录组学反应,以及将这些微藻喂给轮虫(Brachionus plicatilis)的后续效应。在高CO2条件下,由于参与光合作用、卡尔文-本森循环和糖酵解的基因上调,I. galbana表现出更高的光合效率、碳固定和能量代谢。这些分子适应使海藻的生长速度提高了24%,多不饱和脂肪酸(PUFAs)含量提高了8%,其中包括二十二碳六烯酸,从而提高了海藻的营养品质。饲养实验表明,以高CO2条件下生长的galbana为食的轮虫表现出更好的生长和丰富的PUFA谱,突出了高CO2诱导的初级生产者变化提高营养转移效率的潜力。这一研究为植物对高CO2响应的分子机制及其生态意义提供了新的见解。这些发现强调了海洋微藻适应环境变化的能力,并强调了二氧化碳升高对海洋食物网营养和动态的级联效应。
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来源期刊
Limnology and Oceanography
Limnology and Oceanography 地学-海洋学
CiteScore
8.80
自引率
6.70%
发文量
254
审稿时长
3 months
期刊介绍: Limnology and Oceanography (L&O; print ISSN 0024-3590, online ISSN 1939-5590) publishes original articles, including scholarly reviews, about all aspects of limnology and oceanography. The journal''s unifying theme is the understanding of aquatic systems. Submissions are judged on the originality of their data, interpretations, and ideas, and on the degree to which they can be generalized beyond the particular aquatic system examined. Laboratory and modeling studies must demonstrate relevance to field environments; typically this means that they are bolstered by substantial "real-world" data. Few purely theoretical or purely empirical papers are accepted for review.
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