Six Hundred and Sixty Nanometer Light Exposure-Induced Alterations in Actin Filament, Mitochondrial Morphological Dynamics, and Migration in Mesenchymal Stem Cells.

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Journal of biophotonics Pub Date : 2025-09-05 DOI:10.1002/jbio.202400544

Mahima Rastogi, Khageswar Sahu, Shovan Kumar Majumder

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Abstract

Actin cytoskeleton alteration and cell homing/migration are crucial determinants for the success of stem cell (SC) based therapy. Photobiomodulation (PBM) is a promising non-pharmacological approach for modulating SC potency. Though ~660 nm is the most studied wavelength for the proliferation/differentiation of SCs, the migration and cytoskeleton remodeling aspects have not been investigated in detail. In this study, we report the effect of ~660 nm on actin filaments, mitochondrial morphological dynamics, along with the migration of human adipose-derived mesenchymal stem cells (hADMSCs). Exposure to ~660 nm (~15 J/cm²) elicits rapid actin fiber rearrangement leading to elongated, parallel fibers, and mitochondrial granulation along the leading edge of cell migration. In addition, 660 nm (~15 J/cm²) also enhances cell proliferation, ATP, and ROS levels. These ultrastructural and biochemical alterations, in conjunction with the increased cell migration, shed new light on mechanistic perspectives to elicit enhanced homing/migration in SCs and would help in further optimization of ~660 nm based SC priming.

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660纳米光照射诱导间充质干细胞肌动蛋白丝、线粒体形态动力学和迁移的改变。

肌动蛋白细胞骨架改变和细胞归巢/迁移是干细胞治疗成功的关键决定因素。光生物调节（PBM）是一种很有前途的调节SC效价的非药物方法。虽然~660 nm是研究最多的干细胞增殖/分化波长，但尚未对其迁移和细胞骨架重塑方面进行详细研究。在这项研究中，我们报道了~660 nm对肌动蛋白丝、线粒体形态动力学以及人脂肪源性间充质干细胞（hADMSCs）迁移的影响。暴露于~ 660nm （~ 15j /cm2）的环境中，肌动蛋白纤维会快速重排，导致沿细胞迁移的前沿出现细长的平行纤维和线粒体肉芽。此外，660 nm （~15 J/cm2）也能促进细胞增殖、ATP和ROS水平。这些超微结构和生化变化，加上细胞迁移的增加，为诱导SCs增强归巢/迁移的机制视角提供了新的视角，并有助于进一步优化~660 nm的SC启动。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Journal of biophotonics

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