{"title":"A novel lytic Seuratvirus phage CABI-SEA 2301 with broad host range against multidrug-resistant avian pathogenic Escherichia coli","authors":"Siwaporn Bilhman , Sakkarin Lethongkam , Anis Waeoh , Arithat Limsatanun , Jiranan Pattano , Rosesathorn Soontarach , Supayang Piyawan Voravuthikunchai , Supakit Paosen","doi":"10.1016/j.tvjl.2025.106426","DOIUrl":null,"url":null,"abstract":"<div><div>Multidrug-resistant avian pathogenic <em>Escherichia coli</em> (MDR-APEC) causes high mortality in newborn chickens, leading to significant economic losses for poultry industry worldwide. The present study proposes an effective alternative strategy to control APEC infections by using bacteriophage specific to MDR-APEC. Lytic phage CABI-SEA 2301 isolated from a chicken faecal sample collected at a commercial poultry farm using the double layer agar overlay technique was classified as a novel species in the genus <em>Seuratvirus</em>, subfamily Queuovirinae. Genome sequencing unveiled that the selected phage consisted of a double-stranded DNA molecule with 61,452 base pairs and a GC content of 38 %, with no toxins, virulence, and antimicrobial resistance. Phage CABI-SEA 2301 demonstrated a broad host range, lysing 68 % of 50 MDR-APEC clinical isolates obtained from <em>E. coli</em> lesions at poultry farms. A high adsorption rate to the host was exhibited with 77 % within 10 min. One-step growth analysis showed that phage CABI-SEA 2301 had a latent period of 30 min with a burst size of 522 PFU/cell. Phage-infected MDR-APEC isolate CABI-SEA 2349 at the lowest multiplicity of infection of 0.01 showed a significant decrease in the bacterial growth after 4 h post-infection. Scanning electron microscopy further confirmed abnormal cellular morphology after treatment. Moreover, phage CABI-SEA 2301 could effectively inhibit the bacterial biofilm formation. Interestingly, the phage survived in mimic simulated chicken gastrointestinal conditions (96.67 ± 4.71 %) and revealed good stability under temperature between −80–50 ˚C and pH 4–12. The findings highlight the potential of the phage CABI-SEA 2301 as a biocontrol agent against MDR-APEC in the commercial farms.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"314 ","pages":"Article 106426"},"PeriodicalIF":3.1000,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary journal","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1090023325001303","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Multidrug-resistant avian pathogenic Escherichia coli (MDR-APEC) causes high mortality in newborn chickens, leading to significant economic losses for poultry industry worldwide. The present study proposes an effective alternative strategy to control APEC infections by using bacteriophage specific to MDR-APEC. Lytic phage CABI-SEA 2301 isolated from a chicken faecal sample collected at a commercial poultry farm using the double layer agar overlay technique was classified as a novel species in the genus Seuratvirus, subfamily Queuovirinae. Genome sequencing unveiled that the selected phage consisted of a double-stranded DNA molecule with 61,452 base pairs and a GC content of 38 %, with no toxins, virulence, and antimicrobial resistance. Phage CABI-SEA 2301 demonstrated a broad host range, lysing 68 % of 50 MDR-APEC clinical isolates obtained from E. coli lesions at poultry farms. A high adsorption rate to the host was exhibited with 77 % within 10 min. One-step growth analysis showed that phage CABI-SEA 2301 had a latent period of 30 min with a burst size of 522 PFU/cell. Phage-infected MDR-APEC isolate CABI-SEA 2349 at the lowest multiplicity of infection of 0.01 showed a significant decrease in the bacterial growth after 4 h post-infection. Scanning electron microscopy further confirmed abnormal cellular morphology after treatment. Moreover, phage CABI-SEA 2301 could effectively inhibit the bacterial biofilm formation. Interestingly, the phage survived in mimic simulated chicken gastrointestinal conditions (96.67 ± 4.71 %) and revealed good stability under temperature between −80–50 ˚C and pH 4–12. The findings highlight the potential of the phage CABI-SEA 2301 as a biocontrol agent against MDR-APEC in the commercial farms.
期刊介绍:
The Veterinary Journal (established 1875) publishes worldwide contributions on all aspects of veterinary science and its related subjects. It provides regular book reviews and a short communications section. The journal regularly commissions topical reviews and commentaries on features of major importance. Research areas include infectious diseases, applied biochemistry, parasitology, endocrinology, microbiology, immunology, pathology, pharmacology, physiology, molecular biology, immunogenetics, surgery, ophthalmology, dermatology and oncology.