An Automated Device for Pathogen Detection in Milk Integrating Magnetic Nanoprobes with the Lateral-Flow Immunoassay

IF 3 3区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Food Analytical Methods Pub Date : 2025-07-15 DOI:10.1007/s12161-025-02855-2

Humza Y. Malik, Lina N. Kafadarian, Jeeth S. Pawar, Robin B. Shi, Emika G. Saito, Isabel Thomas, Daniel T. Kamei

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引用次数: 0

Abstract

Foodborne illnesses, particularly in milk, pose significant public health risks, especially in low-income regions where access to reliable testing is limited. Lateral-flow immunoassays (LFAs) offer rapid detection but suffer from small volume capacity and low sensitivity. To address these challenges, we developed a fully automated device that utilizes immunomagnetic separation (IMS), where magnetic nanoprobes (MNPs) selectively capture Escherichia coli (E. coli) in large volumes of milk. The device then removes excess sample and adds a buffer to minimize matrix effects before LFA application. Our device achieves a limit of detection (LOD) of 5 × 10³ colony-forming units per milliliter (cfu/mL), a 20-fold improvement over the standard LFA setup. The device is composed of inexpensive 3D-printed materials and hobby electrical components and has a time to result of 40 min, requiring only sample input and a single button press to complete the entire workflow. This novel device offers a widely accessible and user-friendly solution for small-scale milk distributors for mitigating food contamination outbreaks.

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磁性纳米探针与横向流动免疫分析法相结合的牛奶病原体自动检测装置

食源性疾病，特别是牛奶中的食源性疾病，构成重大的公共卫生风险，特别是在获得可靠检测的机会有限的低收入地区。侧流免疫分析法（LFAs）检测速度快，但容量小，灵敏度低。为了解决这些挑战，我们开发了一种利用免疫磁分离（IMS）的全自动装置，其中磁性纳米探针（MNPs）选择性地捕获大量牛奶中的大肠杆菌（E. coli）。该设备然后去除多余的样品，并添加一个缓冲器，以尽量减少LFA应用前的矩阵效应。我们的设备达到了每毫升5 × 103菌落形成单位（cfu/mL）的检测限（LOD），比标准LFA设置提高了20倍。该设备由廉价的3d打印材料和业余电子元件组成，产生结果的时间为40分钟，只需要样品输入和单个按钮按下即可完成整个工作流程。这种新型装置为小型牛奶经销商提供了一种广泛使用和用户友好的解决方案，以减轻食品污染的爆发。

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来源期刊

Food Analytical Methods 农林科学-食品科技

CiteScore

6.00

自引率

3.40%

发文量

244

审稿时长

3.1 months

期刊介绍： Food Analytical Methods publishes original articles, review articles, and notes on novel and/or state-of-the-art analytical methods or issues to be solved, as well as significant improvements or interesting applications to existing methods. These include analytical technology and methodology for food microbial contaminants, food chemistry and toxicology, food quality, food authenticity and food traceability. The journal covers fundamental and specific aspects of the development, optimization, and practical implementation in routine laboratories, and validation of food analytical methods for the monitoring of food safety and quality.