Salvia lanigera Poiret Extracts: Study of the Phytochemical Profiling via GC–MS and HPLC–DAD and Bioactivity with ADME Analysis

IF 3 3区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Food Analytical Methods Pub Date : 2025-07-22 DOI:10.1007/s12161-025-02863-2

Renda Chahna, Hamdi Bendif, Amina Bouzana, Larbi Derbak, Imane Haouame, Dilaycan Çam, Mehmet Öztürk, Khellaf Rebbas, Mohamed A. M. Ali, Chawki Bensouıci, Fehmi Boufahja, Stefania Garzoli

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Abstract

This investigation evaluated the chemical composition and the biological activities of the ethanol and petroleum ether extracts of Salvia lanigera Poiret from the M’sila region, Algeria. Phytochemical analysis identified 17 compounds in the ethanol extract (HPLC–DAD), with cynarin, ellagic acid, and rutin as major components. Petroleum ether extract (GC–MS) revealed 16 compounds, predominantly palmitic acid and stearic acid. Antioxidant activity was assessed using four assays: the ethanol extract showed significant activity in the phenanthroline assay (1.94 ± 0.18 μg/mL), and SNP assay (124.78 ± 0.59 μg/mL), compared to the BHA standard. Both extracts demonstrated antibacterial and antifungal effects, with inhibition zones of 10–13 mm and MIC values ranging from 0.78 to 3.125 mg/mL against tested strains. Enzymatic assays revealed α-glucosidase inhibition by the ethanol extract (IC₅₀ = 27.07 ± 0.78 μg/mL), while α-amylase inhibition was lower (ethanol: 429.85 ± 1.43 μg/mL; petroleum ether: 520.31 ± 1.63 μg/mL). Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibition were minimal (IC₅₀ > 200 μg/mL for AChE; ethanol: 365.84 ± 5.48 μg/mL, petroleum ether: 636.13 ± 4.49 μg/mL for BChE). Urease inhibition was notable for the ethanol extract (54.88%) and comparable for the petroleum ether extract (52.00%). These findings highlight the potential of S. lanigera extracts as sources of bioactive compounds with antioxidant, antimicrobial, and enzymatic inhibitory properties, warranting further exploration for therapeutic applications.

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鼠尾草提取物的GC-MS、HPLC-DAD及ADME生物活性研究

本研究对阿尔及利亚M 'sila地区鼠尾草乙醇提取物和石油醚提取物的化学成分和生物活性进行了研究。植物化学分析鉴定出乙醇提取物中的17种化合物，主要成分为cynarin、鞣花酸和芦丁。石油醚提取物（GC-MS）共鉴定出16种化合物，主要为棕榈酸和硬脂酸。采用四种方法评估其抗氧化活性：与BHA标准相比，乙醇提取物在菲罗啉测定（1.94±0.18 μg/mL）和SNP测定（124.78±0.59 μg/mL）中具有显著活性。两种提取物均具有抑菌抑菌作用，抑菌范围为10 ~ 13 mm， MIC值为0.78 ~ 3.125 mg/mL。酶测结果表明，乙醇提取物对α-葡萄糖苷酶有抑制作用（IC50 = 27.07±0.78 μg/mL），对α-淀粉酶有较低的抑制作用（乙醇：429.85±1.43 μg/mL；石油醚：520.31±1.63 μg/mL）。乙酰胆碱酯酶（AChE）和丁基胆碱酯酶（BChE）对乙酰胆碱酯酶（AChE）的抑制作用最小（IC50 > 200 μg/mL，乙醇：365.84±5.48 μg/mL，石油醚：636.13±4.49 μg/mL）。乙醇提取物的脲酶抑制率为54.88%，石油醚提取物的脲酶抑制率为52.00%。这些发现突出了黑毛藤提取物作为具有抗氧化、抗菌和酶抑制特性的生物活性化合物的潜力，值得进一步探索其在治疗方面的应用。

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来源期刊

Food Analytical Methods 农林科学-食品科技

CiteScore

6.00

自引率

3.40%

发文量

244

审稿时长

3.1 months

期刊介绍： Food Analytical Methods publishes original articles, review articles, and notes on novel and/or state-of-the-art analytical methods or issues to be solved, as well as significant improvements or interesting applications to existing methods. These include analytical technology and methodology for food microbial contaminants, food chemistry and toxicology, food quality, food authenticity and food traceability. The journal covers fundamental and specific aspects of the development, optimization, and practical implementation in routine laboratories, and validation of food analytical methods for the monitoring of food safety and quality.