Impact of nucleation temperature and hydroxyethyl starch on ice crystal growth: Implications for cell viability during extreme temperature fluctuations

IF 2.9 2区 生物学 Q2 BIOLOGY
Nishaka William , Anika Rahman , Jason P. Acker
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引用次数: 0

Abstract

Extreme temperature fluctuations during routine handling and shipping of cryopreserved cell products significantly compromise product quality in ways that extend beyond the duration and peak temperature of the fluctuation. The type of cryoprotectant used and the initial ice nucleation temperature influence ice crystal growth during rewarming events, in turn impacting cell survival. Using a cryomicroscope together with temperature profiles recorded in cord-blood units, ice crystal growth was tracked through five transient-warming events (TWEs) that peaked at −30 °C, −20 °C, or −10 °C. Initial freezing conditions were modified either by adding 6 % (w/v) hydroxyethyl starch (HES) or by lowering the ice-nucleation temperature by 10 °C. Across five TWEs, ice-crystal area saw the greatest increase when the peak rewarming temperature was −10 °C. Although adding HES further accelerated this recrystallization, it still protected Jurkat cells after a single TWE. Lowering the nucleation temperature also improved viability in samples warmed to −20 °C, regardless of HES supplementation. These findings show that ice crystal growth is not the sole cause of injury during transient rewarming; other temperature-dependent stresses also play a role. Importantly, careful optimisation of cryoprotectant composition and nucleation temperature can bolster cellular resilience to temperature excursions, potentially reducing quality losses during the storage and transport of cryopreserved therapeutics.
成核温度和羟乙基淀粉对冰晶生长的影响:极端温度波动对细胞活力的影响
在低温保存细胞产品的日常处理和运输过程中,极端的温度波动会以超出波动持续时间和峰值温度的方式严重损害产品质量。使用的冷冻保护剂类型和初始冰核温度会影响再升温过程中冰晶的生长,进而影响细胞存活。使用低温显微镜和记录在脐带血单位中的温度分布,通过5次瞬时变暖事件(TWEs)来跟踪冰晶生长,这些事件在- 30°C, - 20°C或- 10°C达到峰值。通过加入6% (w/v)的羟乙基淀粉(HES)或将冰核温度降低10℃来改变初始冷冻条件。在5个TWEs中,当再暖峰值温度为- 10°C时,冰晶面积增加最大。虽然加入HES进一步加速了这种再结晶,但在单次TWE后,它仍然可以保护Jurkat细胞。降低成核温度也提高了样品在加热至- 20°C时的活力,无论是否添加HES。这些发现表明,冰晶生长并不是瞬时复温过程中造成损伤的唯一原因;其他与温度相关的压力也起了作用。重要的是,精心优化冷冻保护剂成分和成核温度可以增强细胞对温度漂移的恢复能力,从而潜在地减少冷冻保存疗法在储存和运输过程中的质量损失。
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来源期刊
Journal of thermal biology
Journal of thermal biology 生物-动物学
CiteScore
5.30
自引率
7.40%
发文量
196
审稿时长
14.5 weeks
期刊介绍: The Journal of Thermal Biology publishes articles that advance our knowledge on the ways and mechanisms through which temperature affects man and animals. This includes studies of their responses to these effects and on the ecological consequences. Directly relevant to this theme are: • The mechanisms of thermal limitation, heat and cold injury, and the resistance of organisms to extremes of temperature • The mechanisms involved in acclimation, acclimatization and evolutionary adaptation to temperature • Mechanisms underlying the patterns of hibernation, torpor, dormancy, aestivation and diapause • Effects of temperature on reproduction and development, growth, ageing and life-span • Studies on modelling heat transfer between organisms and their environment • The contributions of temperature to effects of climate change on animal species and man • Studies of conservation biology and physiology related to temperature • Behavioural and physiological regulation of body temperature including its pathophysiology and fever • Medical applications of hypo- and hyperthermia Article types: • Original articles • Review articles
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