Investigating the Role of Biomarkers Using Liquid Biopsy in the Diagnosis of Meningiomas.

Turkish neurosurgery Pub Date : 2025-01-01 DOI:10.5137/1019-5149.JTN.46743-24.3

Hasiba Karimi, Sadaf Mahfooz, Imran Khan, Busra Karacam, Kerime Akdur, Guven Gonen, Elif Burce Elbasan, Mustafa Aziz Hatiboglu

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Abstract

Aim: To evaluate the diagnostic potential of c-MYC, FABP7, GATA4, and MAOB in meningioma patients by analyzing their expression in serum samples.

Material and methods: The study included 20 patients who underwent surgical resection for intracranial meningiomas. Tumor and serum samples were collected during the surgical procedure. Real-time polymerase chain reaction (RT-PCR) was performed to measure the expression of FABP7, GATA4, c-MYC, and MAOB in both tumor tissues and serum samples.

Results: The expression levels of MAOB, c-MYC, and GATA4 were significantly higher in grade 2 meningioma tumor tissues compared to grade 1 tumors (p=0.031, p=0.041, and p=0.003, respectively). Similarly, patients with grade 2 meningiomas had significantly higher MAOB expression in their serum compared to patients with grade 1 meningiomas (p=0.032). In addition, the serum levels of FABP7 and MAOB were significantly higher in meningioma patients compared to healthy controls (p < 0.05).

Conclusion: The findings of this study suggest that FABP7 and MAOB expression in serum may serve as diagnostic markers for meningiomas. However, additional studies with larger cohorts are necessary to validate these results.

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探讨液体活检生物标志物在脑膜瘤诊断中的作用。

目的：脑膜瘤是成人最常见的原发性脑肿瘤。虽然磁共振成像（MRI）通常用于诊断脑膜瘤，但确定肿瘤分级是具有挑战性的。肿瘤分级对于选择最合适的治疗方法和确定手术切除的范围至关重要。除了通过手术切除获得的组织学分析外，没有确定的方法使用液体活检来分级脑膜瘤。虽然有研究检测了c-MYC、FABP7、GATA4和MAOB在肿瘤组织中的表达，但它们在血清中的表达尚未得到充分的探讨。本研究旨在通过分析这些基因在脑膜瘤患者血清样本中的表达来评估其诊断潜力。材料和方法：本研究包括20例颅内脑膜瘤手术切除患者。在手术过程中收集肿瘤和血清样本。采用实时聚合酶链反应（RT-PCR）检测肿瘤组织和血清样品中FABP7、GATA4、c-MYC和MAOB的表达。结果：MAOB、c-MYC、GATA4在2级脑膜瘤组织中的表达水平明显高于1级脑膜瘤组织（p=0.031、p=0.041、p=0.003）。同样，2级脑膜瘤患者血清中MAOB表达明显高于1级脑膜瘤患者（p=0.032）。此外，脑膜瘤患者血清FABP7和MAOB水平显著高于健康对照组（p 0.05）。结论：血清中FABP7和MAOB的表达可作为脑膜瘤的诊断指标。然而，需要更多的研究来验证这些结果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Turkish neurosurgery

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