Detection of human herpesvirus 7 in conjunctival samples collected from individuals recovering from conjunctivitis.

IF 0.9
Frontiers in ophthalmology Pub Date : 2025-08-19 eCollection Date: 2025-01-01 DOI:10.3389/fopht.2025.1641991
Andrew T E Hartwick, Christina E Morettin, Jennifer S Harthan, Meredith Whiteside, Ellen Shorter, Spencer D Johnson, Mary K Migneco, Christian K Olson, Julia B Huecker, Tammy Than, Mae O Gordon
{"title":"Detection of human herpesvirus 7 in conjunctival samples collected from individuals recovering from conjunctivitis.","authors":"Andrew T E Hartwick, Christina E Morettin, Jennifer S Harthan, Meredith Whiteside, Ellen Shorter, Spencer D Johnson, Mary K Migneco, Christian K Olson, Julia B Huecker, Tammy Than, Mae O Gordon","doi":"10.3389/fopht.2025.1641991","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>Although it is often reported that adenovirus is the most common etiology for infectious conjunctivitis, a recent multi-center clinical study found that adenovirus was confirmed by polymerase chain reaction in only 16% of cases presenting with acute conjunctivitis. Here, we investigated the hypothesis that a member of Herpesviridae could be the underlying etiology in some non-adenoviral cases of conjunctivitis.</p><p><strong>Methods: </strong>Molecular assays for Herpes Simplex 1 and 2 (HSV-1, HSV-2) and Human Herpesvirus 6A, 6B and 7 (HHV-6A, HHV-6B, HHV-7) were performed on conjunctival samples collected from 18 individuals with acute conjunctivitis and during their recovery in follow-up visits that spanned up to 3 weeks. All samples, obtained from individuals enrolled in a clinical trial evaluating a conjunctivitis treatment, were from eyes that had previously tested negative for adenovirus using polymerase chain reaction (PCR) techniques.</p><p><strong>Results: </strong>In total, 160 PCR assays were performed on 40 conjunctival samples. Four of these samples, obtained from four different individuals, tested positive for HHV-7. None of the samples tested positive for HSV-1, HSV-2, HHV-6A or HHV-6B.</p><p><strong>Conclusion: </strong>This data provides further evidence that Human Herpesvirus 7 can be present in the eye, as HHV-7 was detected in a subset of conjunctival samples obtained from individuals recovering from non-adenoviral conjunctivitis. Clinicians should consider non-adenoviral etiologies when managing conjunctivitis that presents as classic 'pink eye'.</p>","PeriodicalId":73096,"journal":{"name":"Frontiers in ophthalmology","volume":"5 ","pages":"1641991"},"PeriodicalIF":0.9000,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12401696/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in ophthalmology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3389/fopht.2025.1641991","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Purpose: Although it is often reported that adenovirus is the most common etiology for infectious conjunctivitis, a recent multi-center clinical study found that adenovirus was confirmed by polymerase chain reaction in only 16% of cases presenting with acute conjunctivitis. Here, we investigated the hypothesis that a member of Herpesviridae could be the underlying etiology in some non-adenoviral cases of conjunctivitis.

Methods: Molecular assays for Herpes Simplex 1 and 2 (HSV-1, HSV-2) and Human Herpesvirus 6A, 6B and 7 (HHV-6A, HHV-6B, HHV-7) were performed on conjunctival samples collected from 18 individuals with acute conjunctivitis and during their recovery in follow-up visits that spanned up to 3 weeks. All samples, obtained from individuals enrolled in a clinical trial evaluating a conjunctivitis treatment, were from eyes that had previously tested negative for adenovirus using polymerase chain reaction (PCR) techniques.

Results: In total, 160 PCR assays were performed on 40 conjunctival samples. Four of these samples, obtained from four different individuals, tested positive for HHV-7. None of the samples tested positive for HSV-1, HSV-2, HHV-6A or HHV-6B.

Conclusion: This data provides further evidence that Human Herpesvirus 7 can be present in the eye, as HHV-7 was detected in a subset of conjunctival samples obtained from individuals recovering from non-adenoviral conjunctivitis. Clinicians should consider non-adenoviral etiologies when managing conjunctivitis that presents as classic 'pink eye'.

结膜炎恢复期患者结膜标本中人疱疹病毒7型的检测。
目的:虽然经常报道腺病毒是感染性结膜炎最常见的病因,但最近的一项多中心临床研究发现,只有16%的急性结膜炎病例通过聚合酶链反应证实了腺病毒。在这里,我们调查了疱疹病毒科成员可能是一些非腺病毒结膜炎病例的潜在病因的假设。方法:对18例急性结膜炎患者及康复期结膜标本进行为期3周的单纯疱疹病毒1型和2型(HSV-1、HSV-2)和人类疱疹病毒6A、6B和7型(HHV-6A、HHV-6B、HHV-7)分子检测。所有样本来自参加结膜炎治疗评估临床试验的个体,均来自先前使用聚合酶链反应(PCR)技术对腺病毒检测呈阴性的眼睛。结果:共对40份结膜标本进行了160次PCR检测。从四个不同的个体获得的这些样本中,有四个检测出HHV-7阳性。所有样本均未检测出HSV-1、HSV-2、HHV-6A或HHV-6B阳性。结论:这一数据提供了进一步的证据,证明人类疱疹病毒7可以存在于眼睛中,因为在从非腺病毒结膜炎恢复的个体的结膜样本中检测到HHV-7。临床医生在处理典型的“红眼”结膜炎时应考虑非腺病毒病因。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
0.50
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信