A modular, adaptable, and accessible implant kit for chronic electrophysiological recordings in rats.

IF 4.5 Q1 BIOCHEMICAL RESEARCH METHODS

Cell Reports Methods Pub Date : 2025-09-15 Epub Date: 2025-09-02 DOI:10.1016/j.crmeth.2025.101146

Raquel J Ibáñez Alcalá, Andrea Y Macias, Cory N Heaton, Ricardo Sosa Jurado, Alexis A Salcido, Neftali F Reyes, Serina A Batson, Luis D Davila, Dirk W Beck, Lara I Rakocevic, Atanu Giri, Kenichiro Negishi, Sabrina M Drammis, Ki A Goosens, Travis M Moschak, Alexander Friedman

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Abstract

Electrophysiological implants enable exploration of the relationship between neuronal activity and behavior. These technologies evolve rapidly, with multiple iterations of recording systems developed and utilized. Chronic implants must address a litany of complications, including retention of high signal-to-noise ratio in probes and the ability to withstand excess force over the experimental period. To overcome these issues, we designed a chronic implant for rats. Our comprehensive protocol optimizes the entire implant process, from assembling and testing the probes (Neuropixels) to implantation. In addition to addressing the complications previously mentioned, our implant can vertically adjust probes with micron precision and is constructed using modular components, allowing it to be easily modified for various research contexts, electrophysiological recording systems, headstages, and probe types.

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大鼠慢性电生理记录的模块化，适应性强，易于使用的植入试剂盒。

电生理植入物能够探索神经元活动和行为之间的关系。随着记录系统的多次迭代开发和利用，这些技术发展迅速。慢性植入必须解决一系列的并发症，包括在探针中保持高信噪比和在实验期间承受过大力的能力。为了克服这些问题，我们为大鼠设计了一个慢性植入物。我们的综合方案优化了整个植入过程，从组装和测试探针（Neuropixels）到植入。除了解决前面提到的并发症外，我们的植入物可以垂直调整微米精度的探针，并使用模块化组件构建，使其能够轻松地针对各种研究背景，电生理记录系统，前置级和探针类型进行修改。

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