Inhibition of MMP activity and preservation of dentin collagen structure of zinc-releasing glass ionomer cement

IF 5.5 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of dentistry Pub Date : 2025-09-01 DOI:10.1016/j.jdent.2025.106082

Elseoudy Nader , Sanon Kittisak , Hiraishi Noriko , Tagami Junji , Shimada Yasushi

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引用次数: 0

Abstract

Objective

This study evaluated the ability of a novel zinc-releasing glass ionomer cement (GIC), Caredyne Restore (CR), to inhibit matrix metalloproteinase (MMP) activity and preserve dentin collagen integrity, compared with a conventional GIC (Fuji IX, FIX) and chlorhexidine (CHX), a widely used MMP inhibitor.

Methods

Demineralized bovine dentin beams were allocated to four groups: deionized water (negative control), CHX (positive control), FIX eluate, and CR eluate. MMP Activity was assessed before and after treatment using a colorimetric assay. Dentin matrix degradation was evaluated by measuring dry mass loss over 21 days in simulated body fluid and collagen fragment release after one week using Sirius Red staining.

Results

CR exhibited the greatest reduction in MMP activity (65.5 %), significantly higher than FIX (44.8 %, p = 0.02) and comparable to CHX (61 %). CR also demonstrated the lowest cumulative dry mass loss (4 %), significantly less than the control (45.8 %, p = 0.001) and FIX (p = 0.03), and comparable to CHX (13 %). Collagen fragment release was significantly reduced in the CR group (32.5 %) relative to the control (p < 0.001), comparable to CHX (27.7 %).

Conclusion

CR demonstrated significant inhibition of MMP activity and effective preservation of dentin matrix integrity compared to a conventional GIC.

Clinical significance

The use of CR may help protect dentin collagen from enzymatic degradation, potentially improving the durability and longevity of restorations in caries-affected dentin, especially in cases with a high risk of secondary caries.

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释锌玻璃离子水门汀对MMP活性的抑制及对牙本质胶原结构的保护。

目的：本研究评估了一种新型释锌玻璃离子水泥（GIC）， Caredyne Restore (CR)，与传统的GIC （Fuji IX， FIX）和广泛使用的MMP抑制剂氯己定（CHX）相比，抑制基质金属蛋白酶（MMP）活性和保持牙本质胶原完整性的能力。方法：将脱矿牛牙本质梁分为去离子水组（阴性对照）、CHX组（阳性对照）、FIX洗脱液组和CR洗脱液组。用比色法测定治疗前后的MMP活性。用天狼星红染色法测量模拟体液中21天的干质量损失和一周后胶原碎片的释放，以评估牙本质基质的降解。结果：CR表现出最大的MMP活性降低（65.5%），显著高于FIX (44.8%, p = 0.02)，与CHX（61%）相当。CR也表现出最低的累积干质量损失（4%），显著低于对照组（45.8%,p = 0.001）和FIX (p = 0.03)，与CHX（13%）相当。与对照组相比，CR组（32.5%）的胶原碎片释放明显减少（p < 0.001），与CHX组（27.7%）相当。结论：与传统的GIC相比，CR表现出明显的抑制MMP活性和有效保存牙本质基质完整性。临床意义：CR的使用可能有助于保护牙本质胶原蛋白免受酶降解，潜在地提高龋齿影响牙本质修复的耐久性和寿命，特别是在继发性龋齿高风险的病例中。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of dentistry 医学-牙科与口腔外科

CiteScore

7.30

自引率

11.40%

发文量

349

审稿时长

35 days

期刊介绍： The Journal of Dentistry has an open access mirror journal The Journal of Dentistry: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. The Journal of Dentistry is the leading international dental journal within the field of Restorative Dentistry. Placing an emphasis on publishing novel and high-quality research papers, the Journal aims to influence the practice of dentistry at clinician, research, industry and policy-maker level on an international basis. Topics covered include the management of dental disease, periodontology, endodontology, operative dentistry, fixed and removable prosthodontics, dental biomaterials science, long-term clinical trials including epidemiology and oral health, technology transfer of new scientific instrumentation or procedures, as well as clinically relevant oral biology and translational research. The Journal of Dentistry will publish original scientific research papers including short communications. It is also interested in publishing review articles and leaders in themed areas which will be linked to new scientific research. Conference proceedings are also welcome and expressions of interest should be communicated to the Editor.