The ferrous iron transporter FeoB mediates motility, biofilm formation, and virulence in Aeromonas veronii

IF 3.5 3区医学 Q3 IMMUNOLOGY

Microbial pathogenesis Pub Date : 2025-09-01 DOI:10.1016/j.micpath.2025.108014

Yong-Chao Guan , Shuang Liang , Ying-Da Wang , Shao-Yuan Bai , Che-Bing Huang , Jing-Zhi Gong , Wen-Qian Shi , Yuan-Huan Kang , Xiao-Feng Shan , Si-Yang Huang

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引用次数: 0

Abstract

Aeromonas veronii (A. veronii) is a widespread pathogen that can affect human, animals, and aquatic. The ferrous iron (Fe²⁺) transport system (Feo system) is essential for bacterial survival and virulence. Within this system, FeoB, a transmembrane NTPase, plays a key role in maintaining iron homeostasis. However, the role of feoB in the A. veronii is still not clear. In this study, a stable A. veronii feoB genetic mutant strain (ΔfeoB) was constructed, and a strain complemented this mutation (C-feoB). Compared to the wild-type A. veronii strain (TH0426), ΔfeoB exhibited reduced gentamicin sensitivity and impaired growth under iron-limited conditions. Additionally, the ΔfeoB mutant exhibited significant reduction in motility, adhesion, and invasion capabilities. In term of virulence, the wild-type strain exhibited a 2.62-fold higher cytotoxicity toward EPC cells than ΔfeoB. And the LD₅₀ of ΔfeoB was 178-fold lower than that of the wild-type strain, indicating a substantial attenuation of virulence. Consistently, Carassius infected with ΔfeoB displayed significantly lower bacterial loads in tissues. Moreover, the ΔfeoB exhibited diminished antioxidant capacity. In conclusion, this study initially revealed the role of feoB gene in A. veronii TH0426, and provided a new insight into reducing bacterial infectivity and virulence by targeting the regulation of Feo systems.

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亚铁转运体FeoB介导维氏气单胞菌的运动、生物膜形成和毒力。

维罗尼气单胞菌是一种广泛存在的病原体，可影响人类、动物和水生动物。亚铁（Fe2+）转运系统（Feo系统）对细菌的生存和毒力至关重要。在这个系统中，FeoB，一种跨膜NTPase，在维持铁稳态中起着关键作用。然而，feoB在维罗氏单胞菌中的作用尚不清楚。本研究构建了一株稳定的维罗尼乌菌feoB基因突变菌株（ΔfeoB），并构建了一株补充该突变的菌株（C-feoB）。与野生型veronii菌株（TH0426）相比，ΔfeoB在铁限制条件下表现出庆大霉素敏感性降低和生长受损。此外，ΔfeoB突变体表现出运动性、粘附性和侵袭能力的显著降低。毒力方面，野生型菌株对EPC细胞的毒力比ΔfeoB高2.62倍。与野生型菌株相比，ΔfeoB的LD50降低了178倍，毒力明显减弱。一致地，感染ΔfeoB的鲫鱼在组织中显示出明显较低的细菌负荷。此外，ΔfeoB的抗氧化能力下降。综上所述，本研究初步揭示了feoB基因在veronii TH0426中的作用，为通过调控Feo系统来降低细菌感染性和毒力提供了新的思路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microbial pathogenesis 医学-免疫学

CiteScore

7.40

自引率

2.60%

发文量

472

审稿时长

56 days

期刊介绍： Microbial Pathogenesis publishes original contributions and reviews about the molecular and cellular mechanisms of infectious diseases. It covers microbiology, host-pathogen interaction and immunology related to infectious agents, including bacteria, fungi, viruses and protozoa. It also accepts papers in the field of clinical microbiology, with the exception of case reports. Research Areas Include: -Pathogenesis -Virulence factors -Host susceptibility or resistance -Immune mechanisms -Identification, cloning and sequencing of relevant genes -Genetic studies -Viruses, prokaryotic organisms and protozoa -Microbiota -Systems biology related to infectious diseases -Targets for vaccine design (pre-clinical studies)