Placenta specific 1: a novel marker for detection of metastasis in mouse model of breast cancer.

IF 1.9 4区医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biomarkers Pub Date : 2025-09-10 DOI:10.1080/1354750X.2025.2556166

Sadegh Safaei, Masoumeh Dehghan Manshadi, Hannaneh Golshahi, Farideh Hashemi, Farhang Sasani, Zahra Madjd, Roya Ghods

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引用次数: 0

Abstract

Background: Placental-specific 1 (Plac1), with no expression in normal tissues, is expressed in different cancers. Therefore, the potential application of Plac1 to detect metastasis was investigated in breast cancer model.

Methods: A spontaneous metastasis model was established using 4T1 cells. FDG-PET and histological analysis were used to detect metastasis. Plac1 expression was assessed in a wide range of tumor-bearing and normal mice tissues by RT-qPCR. The sensitivity of Plac1-positive cell detection was examined by 4T1 serial dilution in Plac1-negative cells.

Results: Plac1 was not expressed in normal mouse tissues (n = 6), except in the brain (6/6, dCT = -10.85). 4T1 cell line (dCT = 0.65) and 4T1-induced tumor (dCT = -0.29) were positive for Plac1 expression. PET imaging and histopathology analysis demonstrated metastases in the lung, liver, and spleen of tumor-bearing mice. Plac1 expression was confirmed in lung (6/6, dCT = -1.52), liver (6/6, dCT = -2.37), spleen (6/6, dCT = -3.7), kidney (2/6, dCT = -40.00), brain (6/6, dCT = -7.47), and blood (6/6, dCT = -3.35) of tumor-bearing mice. The sensitivity of detecting tumor cells is at least one cell per million cells.

Conclusions: Plac1 is a novel marker with high specificity and sensitivity for detecting metastasis in breast cancer. These findings provide a rationale for human studies.

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胎盘特异性1：检测乳腺癌小鼠模型转移的新标志物。

背景：Placental-specific 1 （Plac1）在正常组织中不表达，但在不同肿瘤中均有表达。因此，我们研究了Plac1在乳腺癌模型中检测转移的潜在应用。方法：采用4T1细胞建立自发性转移瘤模型。采用FDG-PET和组织学分析检测转移。采用RT-qPCR方法在大范围荷瘤小鼠和正常小鼠组织中检测Plac1的表达。在Plac1阴性细胞中采用4T1连续稀释法检测Plac1阳性细胞的敏感性。结果：除脑组织（6/6,dCT= -10.85）外，正常小鼠组织（n = 6）均无Plac1表达。4T1细胞系（dCT= 0.65）和4T1诱导肿瘤（dCT= -0.29） Plac1表达阳性。PET显像和组织病理学分析显示肿瘤在荷瘤小鼠的肺、肝和脾脏转移。Plac1在荷瘤小鼠肺（6/6,dCT= -1.52）、肝（6/6,dCT= -2.37）、脾（6/6,dCT= -3.7）、肾（2/6,dCT= -40.00）、脑（6/6,dCT= -7.47）、血（6/6,dCT= -3.35）中均有表达。检测肿瘤细胞的灵敏度，至少为百万分之一。结论：Plac1是一种新的乳腺癌转移标志物，具有较高的特异性和敏感性。这些发现为人类研究提供了依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomarkers 医学-毒理学

CiteScore

5.00

自引率

3.80%

发文量

140

审稿时长

3 months

期刊介绍： The journal Biomarkers brings together all aspects of the rapidly growing field of biomarker research, encompassing their various uses and applications in one essential source. Biomarkers provides a vital forum for the exchange of ideas and concepts in all areas of biomarker research. High quality papers in four main areas are accepted and manuscripts describing novel biomarkers and their subsequent validation are especially encouraged: • Biomarkers of disease • Biomarkers of exposure • Biomarkers of response • Biomarkers of susceptibility Manuscripts can describe biomarkers measured in humans or other animals in vivo or in vitro. Biomarkers will consider publishing negative data from studies of biomarkers of susceptibility in human populations.