Generation of an induced pluripotent stem cell line, JHUi008-A, from a healthy female donor

Abstract

The use of well characterized human induced pluripotent stem cells (hiPSCs) is essential for developmental studies and disease modeling. Here, we report the generation of a normal, female line of hiPSCs following reprogramming of peripheral blood mononuclear cells (PBMCs) derived from a healthy female donor using Sendai virus technology. This line, which has been extensively employed for the in vitro study of mesoderm-derived cardiomyocytes, is available and registered in the Human Pluripotent Stem Cell Registry (hPSCreg). It exhibits a normal karyotype, expresses stemness markers, and demonstrates robust trilineage differentiation potential.