OsBRP1 acts as a regulator of rice development and salt stress response rather than canonical general transcription factor

IF 6.8 Q1 PLANT SCIENCES

Plant Stress Pub Date : 2025-08-31 DOI:10.1016/j.stress.2025.101024

Su Yang , Guangna Chen , He Ning , Jialuo Chen , Hao Chen , Siyi Wang , Cheng Zhu , Pei Xu

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引用次数: 0

Abstract

Transcriptional initiation in eukaryotes depends on general transcription factor (GTFs), among which TFIIB serving as a core component of the Pol II pre-initiation complex (PIC) assembly. While most eukaryotes possess only two conserved TFIIB paralogs, plants exhibit remarkable expansion of TFIIB-related proteins, indicating functional divergence. Here, we characterize OsBRP1, a plant-specific TFIIB-related protein in rice, and reveal its critical function in coordinating reproductive development and salt stress tolerance. Expression profiling and GUS staining showed that OsBRP1 is preferentially expressed in reproductive tissues. Subcellular localization analysis revealed a unique Endoplasmic Reticulum (ER) retention mechanism via its N-terminal domain. Yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) indicate that OsBRP1 is not involved in the formation of PIC. CRISPR/Cas9-generated mutants exhibited reduced plant height, grain size, and salt tolerance, while OsBRP1-overexpressing (OE) lines showed enhanced agronomic traits and significant resistance to salt stress. Physiological analyses demonstrated that OsBRP1 modulates proline biosynthesis, suppresses oxidative damage, and maintains Na⁺homeostasis under salinity. RNA-seq analysis revealed that under normal conditions, OsBRP1 could influence DNA repair, detoxification, and auxin signaling. Salt stress amplifies these effects, upregulating stress-responsive genes while downregulating genes related to photosynthesis and ROS scavenging. KEGG enrichment highlighted roles in phenylpropanoid biosynthesis, plant-pathogen interaction, and photosynthesis. Together, our results support that OsBRP1 acts as a regulator of reproductive development and salt stress response rather than canonical general transcription factor, offering new insights into plant gene evolution.

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OsBRP1是水稻发育和盐胁迫反应的调节因子，而不是典型的通用转录因子

真核生物的转录起始依赖于一般转录因子（GTFs），其中TFIIB是Pol II预起始复合物（PIC）组装的核心组分。虽然大多数真核生物只有两个保守的TFIIB类似物，但植物表现出显著的TFIIB相关蛋白扩增，表明功能分化。在这里，我们对水稻中植物特异性tfiib相关蛋白OsBRP1进行了表征，并揭示了其在协调生殖发育和盐胁迫耐受中的关键功能。表达谱和GUS染色显示，OsBRP1优先在生殖组织中表达。亚细胞定位分析揭示了一种独特的内质网（ER）保留机制，通过其n端结构域。酵母双杂交（Y2H）和双分子荧光互补（BiFC）表明OsBRP1不参与PIC的形成。CRISPR/ cas9产生的突变体表现出植株高度、晶粒大小和耐盐性降低，而osbrp1过表达（OE）系表现出增强的农艺性状和对盐胁迫的显著抗性。生理分析表明，OsBRP1调节脯氨酸生物合成，抑制氧化损伤，并在盐度下维持Na+稳态。RNA-seq分析显示，在正常情况下，OsBRP1可以影响DNA修复、解毒和生长素信号传导。盐胁迫放大了这些效应，上调应激反应基因，下调与光合作用和活性氧清除相关的基因。KEGG富集在苯丙类生物合成、植物-病原体相互作用和光合作用中发挥了重要作用。总之，我们的研究结果支持OsBRP1作为生殖发育和盐胁迫反应的调节因子，而不是典型的一般转录因子，为植物基因进化提供了新的见解。

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来源期刊

Plant Stress PLANT SCIENCES-

CiteScore

5.20

自引率

8.00%

发文量

审稿时长

63 days

期刊介绍： The journal Plant Stress deals with plant (or other photoautotrophs, such as algae, cyanobacteria and lichens) responses to abiotic and biotic stress factors that can result in limited growth and productivity. Such responses can be analyzed and described at a physiological, biochemical and molecular level. Experimental approaches/technologies aiming to improve growth and productivity with a potential for downstream validation under stress conditions will also be considered. Both fundamental and applied research manuscripts are welcome, provided that clear mechanistic hypotheses are made and descriptive approaches are avoided. In addition, high-quality review articles will also be considered, provided they follow a critical approach and stimulate thought for future research avenues. Plant Stress welcomes high-quality manuscripts related (but not limited) to interactions between plants and: Lack of water (drought) and excess (flooding), Salinity stress, Elevated temperature and/or low temperature (chilling and freezing), Hypoxia and/or anoxia, Mineral nutrient excess and/or deficiency, Heavy metals and/or metalloids, Plant priming (chemical, biological, physiological, nanomaterial, biostimulant) approaches for improved stress protection, Viral, phytoplasma, bacterial and fungal plant-pathogen interactions. The journal welcomes basic and applied research articles, as well as review articles and short communications. All submitted manuscripts will be subject to a thorough peer-reviewing process.