"Neospora caninum detection in bovine aborted fetuses submitted to the Veterinary Pathology Laboratory (UFMT), Brazil"

IF 1.4 Q3 PARASITOLOGY

Veterinary parasitology, regional studies and reports Pub Date : 2025-09-01 DOI:10.1016/j.vprsr.2025.101331

Katiane dos Santos Hofmeister , Luis Jhordy Alfaro Quillas , Wuglenya Daislla Martins da Silva , Larissa Sabino Pinho Moura , Marco Túlio dos Santos Costa , Luciano Nakazato , John F. Mee , Marcos de Almeida Souza , Caroline Argenta Pescador

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Abstract

Neospora caninum (NC) is a major cause of abortion and neonatal death in cattle worldwide. A prospective, observational diagnostic study was conducted at the Veterinary Laboratory of UFMT to determine the prevalence of this parasite by PCR and immunohistochemistry (IHC) and the associated histopathological lesions in bovine aborted fetuses and placentas. Between 2012 and 2024, 192 bovine abortion cases from mainly dairy and beef farms were investigated. These ranged in estimated fetal age from 3 to 8 months with the majority in the second trimester. All were tested for the presence of N. caninum using a PCR on brain tissue. The 43 (22.4 %) cases which were N. caninum PCR-positive were further examined by IHC and histopathology. Thirty (69) PCR-positive fetuses had histopathological lesions compatible with protozoan infection following ‘blinded’ examination. The most frequent histological lesion was non-suppurative encephalitis, observed in 66.7 % of lesion-positive cases. Of the 11 fetuses that had a liquid consistency in the brain, making it difficult to observe microscopic lesions, 7 (63.7 %) had non-suppurative inflammatory lesions in the skeletal muscle suggesting that skeletal muscle is an excellent auxiliary organ for the diagnosis of Neospora caninum due to its low capacity for autolysis. Immunohistochemistry was positive for Neospora caninum in 10 fetuses (33.33 % - 10/30) with lesions compatible with protozoal infection; this indicates IHC is a relatively insensitive technique.

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提交巴西兽医病理实验室（UFMT）的牛流产胎儿的犬新孢子虫检测

犬新孢子虫（NC）是全世界牛流产和新生儿死亡的主要原因。在UFMT兽医实验室进行了一项前瞻性，观察性诊断研究，通过PCR和免疫组织化学（IHC）以及牛流产胎儿和胎盘中相关的组织病理学病变来确定这种寄生虫的患病率。2012年至2024年间，调查了192例牛流产病例，主要来自奶牛场和肉牛场。估计胎龄从3到8个月不等，大多数发生在妊娠中期。所有人都用脑组织上的PCR检测了犬链球菌的存在。43例（22.4%）犬奈瑟菌pcr阳性，进一步进行免疫组化和组织病理学检查。在“盲法”检查后，30（69）例pcr阳性胎儿的组织病理学病变与原生动物感染相符。最常见的组织学病变为非化脓性脑炎，占病变阳性病例的66.7%。11例胎儿脑内呈液体状，难以在显微镜下观察病变，其中7例（63.7%）骨骼肌有非化脓性炎症病变，提示骨骼肌由于自溶能力低，是诊断犬新孢子虫的一个很好的辅助器官。10例（33.33% - 10/30）胎儿免疫组化阳性，病变符合原虫感染；这表明免疫组化是一种相对不敏感的技术。

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来源期刊

Veterinary parasitology, regional studies and reports Multiple-

CiteScore

2.90

自引率

7.10%

发文量

126

审稿时长

97 days

期刊介绍： Veterinary Parasitology: Regional Studies and Reports focuses on aspects of veterinary parasitology that are of regional concern, which is especially important in this era of climate change and the rapid and often unconstrained travel of people and animals. Relative to regions, this journal will accept papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites within the field of veterinary medicine. Also, case reports will be considered as they add to information related to local disease and its control; such papers must be concise and represent appropriate medical intervention. Papers on veterinary parasitology from wildlife species are acceptable, but only if they relate to the practice of veterinary medicine. Studies on vector-borne bacterial and viral agents are suitable, but only if the paper deals with vector transmission of these organisms to domesticated animals. Studies dealing with parasite control by means of natural products, both in vivo and in vitro, are more suited for one of the many journals that now specialize in papers of this type. However, due to the regional nature of much of this research, submissions may be considered based upon a case being made by the author(s) to the Editor. Circumstances relating to animal experimentation must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences (obtainable from: Executive Secretary C.I.O.M.S., c/o W.H.O., Via Appia, CH-1211 Geneva 27, Switzerland).