Mucuna atropurpurea seed extract attenuates inflammation by modulation of inflammatory cytokine response and improving the antioxidant system in experimentally-induced carrageenan in rats

Abstract

The present study used Mucuna atropurpurea seed extract 7 days’ pre-treatment to explore the anti-inflammatory potential in Crgn induced acute paw swelling in rats. The induced inflammation and the formation of oedema were determined by measurement of the paw thickness by digital Vernier caliper. An inflammatory response characterized by oedema, cellular infiltration of neutrophils, and levels of anti-oxidant and pro-inflammatory cytokines imbalance. The findings indicate that MASE pre-treatment significantly modulated the local inflammatory response, as evidenced by a marked reduction in paw oedema in rats. Furthermore, MASE pre-treatment resulted in a substantial attenuation of antioxidant status, highlighting its potent antioxidant properties. Moreover, MASE effectively mitigated the impaired inflammatory response following carrageenan injection, as demonstrated by a reduction in the expression of pro-inflammatory cytokines and mediators. Additionally, MASE enhanced the expression of the anti-inflammatory cytokine IL-10. An acute toxicity assessment was also conducted to evaluate the safety profile of MASE, confirming its safety at the tested doses. Such findings underscore the potential therapeutic benefits of MASE in ameliorating oxidative stress associated with inflammatory conditions.