Photonics Detection of Molecular-Specific Spatial Structural Alterations of Cells due to Chronic Alcoholism and Probiotics Treatments in Colon Cancer.

IF 2.3

Journal of biophotonics Pub Date : 2025-08-28 DOI:10.1002/jbio.202500221

Ishmael Apachigawo, Pradeep K Shukla, Dhruvil Solanki, Santanu Maity, Radhakrishna Rao, Prabhakar Pradhan

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Abstract

Photonics/light localization techniques are essential in understanding the structural changes in biological cells/tissues at the nano- to sub-micron scale. It is now known that structural alterations begin at the nanoscale, marking the onset of cancer progression. This photonics study examines the molecular-specific nano-structural alterations of chronic alcoholism and probiotic effects on colon cancer using a mouse model of colon cancer. We assessed alcohol-treated and azoxymethane (AOM) with dextran sulfate sodium (DSS)-induced colitis models, including ethanol (EtOH) and probiotic (L.casei) treatments separately and together. The confocal images were analyzed using the mesoscopic physics-based Inverse Participation Ratio (IPR) technique to quantify structural alterations at nano- to submicron scales. Significant enhancement of cancer progression was observed in the EtOH-treated group, and probiotic treatment with EtOH showed substantial reversal of these changes in colon cancer, underscoring the potential of the IPR technique in detecting and monitoring cancer progression.

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结肠癌慢性酒精中毒和益生菌治疗引起的细胞分子特异性空间结构改变的光子检测。

光子学/光定位技术对于理解生物细胞/组织在纳米到亚微米尺度上的结构变化至关重要。现在我们知道，结构改变始于纳米级，标志着癌症进展的开始。本光子学研究利用结肠癌小鼠模型研究慢性酒精中毒和益生菌对结肠癌的分子特异性纳米结构改变。我们用葡聚糖硫酸钠（DSS）诱导的结肠炎模型评估了酒精处理和偶氮氧甲烷（AOM），包括乙醇（EtOH）和益生菌（L.casei）单独和共同处理。使用基于介观物理的逆参与比（IPR）技术对共聚焦图像进行分析，以量化纳米到亚微米尺度上的结构变化。在EtOH治疗组中观察到癌症进展的显著增强，而益生菌治疗EtOH在结肠癌中显示出这些变化的实质性逆转，强调了IPR技术在检测和监测癌症进展方面的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Journal of biophotonics

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