Biocompatible Pickering Emulsions from Andrias davidianus Byproducts for Promoting Burn Wound Healing.

Abstract

Biological dressings have emerged as a promising approach for effective wound treatment. However, despite extensive research, the fabrication of biomass-based dressings with antioxidant and anti-inflammatory properties, as well as high biocompatibility, remains a challenge. In this study, the byproducts of Andrias davidianus as raw materials were used to prepare a biomass-based Pickering emulsion. A stable emulsion was formed by homogenizing A. davidianus collagen (AD-SC) with liver oil (AD-LO). The antioxidant peptides (AD-BP) were then incorporated into the mixture, and a Pickering emulsion loaded with antioxidant peptides was successfully prepared. The stability of AD-PE was confirmed through storage, centrifugation, and ζ-potential analyses, and the emulsion exhibited the controlled release of the peptides. In vitro experiments confirmed that AD-PE exhibited marked antioxidant activity and high biocompatibility, with no cytotoxicity, and the promotion of cell migration. In addition, in vivo evaluations demonstrated that AD-PE accelerated wound healing by leveraging the synergistic effects of its components to reduce inflammation and mitigate oxidative damage. This work offers a novel approach for the biomedical application of AD-PE and a new strategy for the utilization of A. davidianus processing byproducts.