Jared Morse, Prasanna Ganesh, Kathrine Cowart, Gabriella Ballestas, Fung Kit Tang and Kaho Leung
{"title":"Development of a selective-iodide indicator for live-cell imaging and evaluation of CFTR activity","authors":"Jared Morse, Prasanna Ganesh, Kathrine Cowart, Gabriella Ballestas, Fung Kit Tang and Kaho Leung","doi":"10.1039/D5SD00086F","DOIUrl":null,"url":null,"abstract":"<p >Cystic fibrosis (CF) arises from mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. Monitoring <small>I</small><small><sup><small>−</small></sup></small> transport serves as a critical approach for evaluating CFTR function in live cells, providing a foundation for the development of diagnostic tools and therapeutic treatments. Here, we report an iridium(<small>III</small>) complex (<strong>I-Sense</strong>) for the selective and pH-independent imaging of intracellular <small>I</small><small><sup><small>−</small></sup></small>. By tracking cellular iodide <small>I</small><small><sup><small>−</small></sup></small> uptake, <strong>I-Sense</strong> facilitates the evaluation of CFTR activity in live cells, providing a valuable tool for the functional characterization of CFTR activity.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 10","pages":" 833-838"},"PeriodicalIF":4.1000,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12394795/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Sensors & diagnostics","FirstCategoryId":"1085","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2025/sd/d5sd00086f","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Cystic fibrosis (CF) arises from mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. Monitoring I− transport serves as a critical approach for evaluating CFTR function in live cells, providing a foundation for the development of diagnostic tools and therapeutic treatments. Here, we report an iridium(III) complex (I-Sense) for the selective and pH-independent imaging of intracellular I−. By tracking cellular iodide I− uptake, I-Sense facilitates the evaluation of CFTR activity in live cells, providing a valuable tool for the functional characterization of CFTR activity.
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