{"title":"Development of a selective-iodide indicator for live-cell imaging and evaluation of CFTR activity.","authors":"Jared Morse, Prasanna Ganesh, Kathrine Cowart, Gabriella Ballestas, Fung Kit Tang, Kaho Leung","doi":"10.1039/d5sd00086f","DOIUrl":null,"url":null,"abstract":"<p><p>Cystic fibrosis (CF) arises from mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. Monitoring I<sup>-</sup> transport serves as a critical approach for evaluating CFTR function in live cells, providing a foundation for the development of diagnostic tools and therapeutic treatments. Here, we report an iridium(iii) complex (<b>I-Sense</b>) for the selective and pH-independent imaging of intracellular I<sup>-</sup>. By tracking cellular iodide I<sup>-</sup> uptake, <b>I-Sense</b> facilitates the evaluation of CFTR activity in live cells, providing a valuable tool for the functional characterization of CFTR activity.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" ","pages":""},"PeriodicalIF":4.1000,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12394795/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Sensors & diagnostics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1039/d5sd00086f","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Cystic fibrosis (CF) arises from mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. Monitoring I- transport serves as a critical approach for evaluating CFTR function in live cells, providing a foundation for the development of diagnostic tools and therapeutic treatments. Here, we report an iridium(iii) complex (I-Sense) for the selective and pH-independent imaging of intracellular I-. By tracking cellular iodide I- uptake, I-Sense facilitates the evaluation of CFTR activity in live cells, providing a valuable tool for the functional characterization of CFTR activity.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
