Proteomics-based monitoring of heatstroke recovery identifies molecular signatures of organ stress.

IF 5.4 Q1 MEDICINE, RESEARCH & EXPERIMENTAL
Jiayi Jin, Sing Ying Wong, Kenta Kondo, Toshiaki Iba, Nobuhiro Hayashi
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Abstract

Background: Global warming exacerbates heatstroke, increasing its severity and associated health risks, including fatal kidney damage. Predicting post-heatstroke organ injury remains difficult, delaying timely medical intervention. This study aims to identify potential blood biomarkers that reflect organ stress and recovery status following heatstroke.

Methods: Plasma samples (n = 12) from clinically diagnosed classical (non-exertional) heatstroke patients were collected at diagnosis and recovery. Two-dimensional gel electrophoresis was used to analyze protein expression, identifying 359 protein spots. Selected proteins showing differential expression were validated by Western blotting.

Results: Here, five proteins-alpha-1 antitrypsin, alpha-1 microglobulin/bikunin precursor, apolipoprotein A-IV, clusterin, and complement component 2-show significant changes between the two timepoints. These proteins are linked to inflammatory, coagulation, and lipid metabolism pathways. Alpha-1 antitrypsin, alpha-1 microglobulin, and complement component 2 may reflect the resolution of inflammation, while apolipoprotein A-IV and clusterin indicate renal stress. The alpha-1 microglobulin-IgA complex may exert anti-inflammatory effects. Complement component 2, an initiator of the complement cascade, has not been previously reported to be associated with heat stress.

Conclusions: The findings suggest that these proteins may serve as blood biomarkers to assess heatstroke severity and monitor recovery. Their clinical application could improve early detection of organ damage and guide intervention strategies.

基于蛋白质组学的中暑恢复监测识别器官应激的分子特征。
背景:全球变暖加剧了中暑,增加了其严重程度和相关的健康风险,包括致命的肾脏损害。预测中暑后的器官损伤仍然很困难,延误了及时的医疗干预。本研究旨在确定反映中暑后器官压力和恢复状态的潜在血液生物标志物。方法:收集临床诊断的经典(非运动性)中暑患者在诊断和康复时的血浆样本(n = 12)。双向凝胶电泳分析蛋白表达,鉴定出359个蛋白点。选择表达差异的蛋白进行Western blotting验证。结果:α -1抗胰蛋白酶、α -1微球蛋白/bikunin前体、载脂蛋白A-IV、聚类蛋白和补体成分2在两个时间点之间有显著变化。这些蛋白质与炎症、凝血和脂质代谢途径有关。α -1抗胰蛋白酶、α -1微球蛋白和补体成分2可能反映炎症的消退,而载脂蛋白A-IV和聚簇素可能反映肾脏应激。α -1微球蛋白- iga复合物可能具有抗炎作用。补体成分2是补体级联的启动器,以前没有报道与热应激有关。结论:研究结果表明,这些蛋白可以作为血液生物标志物来评估中暑严重程度和监测恢复情况。它们的临床应用可以提高器官损伤的早期发现和指导干预策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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