Stimulation of the production of proteases from Penicillium verrucosum isolated from Egyptian soil.

Q2 Agricultural and Biological Sciences
Brazilian Journal of Biology Pub Date : 2025-09-01 eCollection Date: 2025-01-01 DOI:10.1590/1519-6984.296244
A A Garziz, M Helal, A M Younis, M A El-Badry, M Yosri
{"title":"Stimulation of the production of proteases from Penicillium verrucosum isolated from Egyptian soil.","authors":"A A Garziz, M Helal, A M Younis, M A El-Badry, M Yosri","doi":"10.1590/1519-6984.296244","DOIUrl":null,"url":null,"abstract":"<p><p>The uses of proteolytic enzymes in manufacturing procedures are numerous. Researchers are investigating a number of strategies to find, rework, or artificially produce enzymes with improved suitability for manufacturing processes in light of the growing needs and uses. Alkaline protease production was assessed in fungal strains that were obtained from soil using the serial dilution technique. Using molecular techniques, the isolate with the greatest potential for producing enzymes was identified. Gel filtering was used to purify the enzyme. In order to enhance fugus ability to generate protease under various growth conditions, this investigation explains how to optimize a culture medium. The fungus with the highest probability was identified as Penicillium verrucosum with accession number PV529631. The purified homodimer protein for purified protease was assessed to have two peaks at 42.5 kDa and 83.7 kDa, consequently upon screening at 280 nm. The optimal culture conditions have been detected upon culture for the isolated fungus at pH =9, temperature = 35 °C, using sucrose as a carbon source, insertion of peptone as a nitrogen and using Soy cake as waste material for culture conditions. The results of the research demonstrate the P. verrucosum strain's capacity to produce the protease enzyme. To fully use the possibilities of these substrates and promote the creation of superior and effective hydrolytic enzymes, it is imperative to keep investigating and refining these approaches.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e296244"},"PeriodicalIF":0.0000,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Brazilian Journal of Biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1590/1519-6984.296244","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 0

Abstract

The uses of proteolytic enzymes in manufacturing procedures are numerous. Researchers are investigating a number of strategies to find, rework, or artificially produce enzymes with improved suitability for manufacturing processes in light of the growing needs and uses. Alkaline protease production was assessed in fungal strains that were obtained from soil using the serial dilution technique. Using molecular techniques, the isolate with the greatest potential for producing enzymes was identified. Gel filtering was used to purify the enzyme. In order to enhance fugus ability to generate protease under various growth conditions, this investigation explains how to optimize a culture medium. The fungus with the highest probability was identified as Penicillium verrucosum with accession number PV529631. The purified homodimer protein for purified protease was assessed to have two peaks at 42.5 kDa and 83.7 kDa, consequently upon screening at 280 nm. The optimal culture conditions have been detected upon culture for the isolated fungus at pH =9, temperature = 35 °C, using sucrose as a carbon source, insertion of peptone as a nitrogen and using Soy cake as waste material for culture conditions. The results of the research demonstrate the P. verrucosum strain's capacity to produce the protease enzyme. To fully use the possibilities of these substrates and promote the creation of superior and effective hydrolytic enzymes, it is imperative to keep investigating and refining these approaches.

从埃及土壤中分离的疣状青霉对蛋白酶产生的刺激作用。
蛋白水解酶在生产过程中的应用是很多的。根据不断增长的需求和用途,研究人员正在研究许多策略,以寻找、重新加工或人工生产具有改进制造过程适用性的酶。利用连续稀释技术对从土壤中获得的真菌菌株的碱性蛋白酶产量进行了评估。利用分子技术鉴定出最具产酶潜力的分离物。采用凝胶过滤法纯化酶。为了提高真菌在各种生长条件下产生蛋白酶的能力,本研究阐述了如何优化培养基。概率最高的真菌为疣状青霉(Penicillium verrucosum),编号PV529631。纯化后的蛋白酶同型二聚体蛋白在280 nm处筛选,在42.5 kDa和83.7 kDa处有两个峰。对分离真菌在pH =9、温度= 35℃、蔗糖为碳源、蛋白胨为氮源、豆饼为废弃物的培养条件下进行了最佳培养条件的检测。研究结果表明,疣状假单胞菌具有生产蛋白酶的能力。为了充分利用这些底物的潜力,促进高效水解酶的产生,必须对这些方法进行不断的研究和完善。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
2.40
自引率
0.00%
发文量
301
审稿时长
4-8 weeks
期刊介绍: The BJB – Brazilian Journal of Biology® is a scientific journal devoted to publishing original articles in all fields of the Biological Sciences, i.e., General Biology, Cell Biology, Evolution, Biological Oceanography, Taxonomy, Geographic Distribution, Limnology, Aquatic Biology, Botany, Zoology, Genetics, and Ecology. Priority is given to papers presenting results of researches in the Neotropical region. Material published includes research papers, review papers (upon approval of the Editorial Board), notes, book reviews, and comments.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信